EC Number   |
Protein Variants |
Reference |
|---|
    6.1.1.2 | A310W |
T2-TrpRS mutant, AMP pocket is blocked, angiostatic activity involves the tryptophan and adenosine pockets |
705874 |
| 6.1.1.2 | A7D |
site-directed mutagenesis, the mutant shows reduced induction of TNF-alpha and MIP-1alpha production compared to wild-type |
745868 |
| 6.1.1.2 | D146A |
site-directed mutagensis, the mutant shows highly reduced activity compared to the wild-type enzyme |
675928 |
| 6.1.1.2 | D382-TIEEHR-Q389 |
deletion of the tRNA anticodon-binding domain insertion, consisting of eight residues in the human TrpRS, abolishes the apoptotic activity of the enzyme for endothelial cells, whereas its translational catalysis and cell-binding activities remain unchanged |
662935 |
| 6.1.1.2 | D99A |
site-directed mutagenesis, the mutant shows reduced kcat and activity compared to the wild-type enzyme |
676210 |
| 6.1.1.2 | D99E |
site-directed mutagenesis, the mutant shows slightly reduced activity and kcat compared to the wild-type enzyme |
676210 |
| 6.1.1.2 | D99K |
site-directed mutagenesis, the mutant shows reduced kcat and activity compared to the wild-type enzyme |
676210 |
| 6.1.1.2 | D99V |
site-directed mutagenesis, the mutant shows reduced kcat and activity compared to the wild-type enzyme |
676210 |
| 6.1.1.2 | E11L |
site-directed mutagenesis, the mutant shows reduced induction of TNF-alpha and MIP-1alpha production compared to wild-type |
745868 |
| 6.1.1.2 | E153D |
ratio of turnover number to Km-value for ATP is 109% of wild-type value, ratio of turnover number to Km-value for Trp is 114% of wild-type value, ratio of turnover number to Km-value for Bacillus subtilis tRNATrp is 89% of wild-type value |
662273 |
