Information on EC 6.1.1.2 - tryptophan-tRNA ligase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
6.1.1.2
-
RECOMMENDED NAME
GeneOntology No.
tryptophan-tRNA ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-tryptophan + tRNATrp = AMP + diphosphate + L-tryptophyl-tRNATrp
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Aminoacylation
esterification
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tRNA charging
-
-
tryptophan metabolism
-
-
Aminoacyl-tRNA biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
L-tryptophan:tRNATrp ligase (AMP-forming)
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-44-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene trpS
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
contains a single TrpRS gene
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
single copy gene
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene trpS
-
-
Manually annotated by BRENDA team
purified enzyme
-
-
Manually annotated by BRENDA team
strain ATCC 12228; strains ATCC 12228 and ATCC 35984
SwissProt
Manually annotated by BRENDA team
gene trpRS1, an auxiliary, antibiotic-resistant tryptophanyl-tRNA synthetase gene
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2-azatryptophan + tRNATrp
AMP + diphosphate + 2-azatryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4,5,6,7-tetrafluorotryptophan + tRNATrp
AMP + diphosphate + 4,5,6,7-tetrafluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-aminotryptophan + tRNATrp
AMP + diphosphate + 4-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-fluorotryptophan + tRNATrp
AMP + diphosphate + 4-fluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-hydroxytryptophan + tRNATrp
AMP + diphosphate + 4-hydroxytryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 4-methyltryptophan + tRNATrp
AMP + diphosphate + 4-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-aminotryptophan + tRNATrp
AMP + diphosphate + 5-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-bromotryptophan + tRNATrp
AMP + diphosphate + 5-bromotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-fluorotryptophan + tRNATrp
AMP + diphosphate + 5-fluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-hydroxy-L-tryptophan + tRNATrp
AMP + diphosphate + 5-hydroxy-L-tryptophan-tRNATrp
show the reaction diagram
-
mutant enzyme V144P selectively aminoacylates the cognate mutant opal suppressor tRNATrp(UCA) with 5-hydroxy-L-tryptophan and not with any endogenous amino acid
-
-
?
ATP + 5-hydroxytryptophan + tRNATrp
AMP + diphosphate + 5-hydroxytryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-iodotryptophan + tRNATrp
AMP + diphosphate + 5-iodotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-methoxytryptophan + tRNATrp
AMP + diphosphate + 5-methoxytryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 5-methyltryptophan + tRNATrp
AMP + diphosphate + 5-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 6-aminotryptophan + tRNATrp
AMP + diphosphate + 6-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 6-fluorotryptophan + tRNATrp
AMP + diphosphate + 6-fluorotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 6-methyltryptophan + tRNATrp
AMP + diphosphate + 6-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 7-aminotryptophan + tRNATrp
AMP + diphosphate + 7-aminotryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 7-azatryptophan + tRNATrp
AMP + diphosphate + 7-azatryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + 7-methyltryptophan + tRNATrp
AMP + diphosphate + 7-methyltryptophyl-tRNATrp
show the reaction diagram
-
-
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73, wild-type)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73C)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73G)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73G)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Archeoglobus fulgidus tRNATrp(A73U)
AMP + diphosphate + Archeoglobus fulgidus L-tryptophanyl-tRNATrp(A73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73, wild-type)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73C)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73G)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73G)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + Bacillus subtilis tRNATrp(A73U)
AMP + diphosphate + Bacillus subtilis L-tryptophanyl-tRNATrp(A73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73, wild-type)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73, wild-type)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73A)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73A)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73C)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73C)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + bovine tRNATrp(G73U)
AMP + diphosphate + bovine L-tryptophanyl-tRNATrp(G73U)
show the reaction diagram
preference profile regarding the discriminator base 73 of different tRNA(Trp) substrates in the order of decreasing efficiency is A, C, U, G. Modest preferences for A73 tRNA(Trp)
-
-
?
ATP + L-tryptophan + tRNAArg
AMP + diphosphate + L-tryptophyl-tRNAArg
show the reaction diagram
-
-
-
-
?
ATP + L-tryptophan + tRNATrp
?
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophan-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophanyl-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophyl-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp(A36C)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36C)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A36G)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A36U)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A36U, A73G)
AMP + diphosphate + L-tryptophyl-tRNATrp(A36U, A73G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A73C)
AMP + diphosphate + L-tryptophyl-tRNATrp(A73C)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A73G)
AMP + diphosphate + L-tryptophyl-tRNATrp(A73G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(A73U)
AMP + diphosphate + L-tryptophyl-tRNATrp(A73U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C34A)
AMP + diphosphate + L-tryptophyl-tRNATrp(C34A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C34G)
AMP + diphosphate + L-tryptophyl-tRNATrp(C34G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C34U)
AMP + diphosphate + L-tryptophyl-tRNATrp(C34U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C35A)
AMP + diphosphate + L-tryptophyl-tRNATrp(C35A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C35G)
AMP + diphosphate + L-tryptophyl-tRNATrp(C35G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(C35U)
AMP + diphosphate + L-tryptophyl-tRNATrp(C35U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G1A-C72U)
AMP + diphosphate + L-tryptophyl-tRNATrp(G1A-C72U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G1C-G72G)
AMP + diphosphate + L-tryptophyl-tRNATrp(G1C-G72G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G1U-C72A)
AMP + diphosphate + L-tryptophyl-tRNATrp(G1U-C72A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G2A-C71U)
AMP + diphosphate + L-tryptophyl-tRNATrp(G2A-C71U)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G2C-G71G)
AMP + diphosphate + L-tryptophyl-tRNATrp(G2C-G71G)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(G2U-C71A)
AMP + diphosphate + L-tryptophyl-tRNATrp(G2U-C71A)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(HOG1)
AMP + diphosphate + L-tryptophyl-tRNATrp(HOG1)
show the reaction diagram
-
substrate mutant
-
-
?
ATP + L-tryptophan + tRNATrp(pppG1)
AMP + diphosphate + L-tryptophyl-tRNATrp(pppG1)
show the reaction diagram
-
substrate mutant
-
-
?
L-tryptophan + ATP
L-Trp-adenylate + diphosphate
show the reaction diagram
the enzyme also catalyzes the exchange of diphosphate in the diphosphate-ATP exchange assay
-
r
L-tryptophan + ATP + ADP
P1,P3-bis(5'-adenosyl)triphosphate + ?
show the reaction diagram
-
-
-
-
L-tryptophan + ATP + NH3
L-tryptophanamide + AMP + diphosphate
show the reaction diagram
-
-
-
-
P1,P3-bis(5'-adenosyl)triphosphate + L-tryptophan + tRNATrp
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-tryptophan + tRNATrp
?
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophan-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophanyl-tRNATrp
show the reaction diagram
ATP + L-tryptophan + tRNATrp
AMP + diphosphate + L-tryptophyl-tRNATrp
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
indolmycin and ATP form a ternary complex with BsTrpRS
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
-
optimal concentration: 50 mM
Mn2+
-
replacing Mg2+ with Mn2+ reduces the specificity for L-tryptophan, increasing the relative activation of L-tyrosine by about 3.5fold
additional information
-
no effects by Fe2+ and diphosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2'-O-methyladenosine
-
-
2-[5-[(6-(ethoxycarbonyl)-5-(4-fluorophenyl)-3-oxo-7-phenyl-5H-[1,3]thiazolo[3,2-a]pyrimidin-2(3H)-ylidene)methyl]-2-furyl]benzoic acid
-
3'-O-Methyl-ATP
-
ATP-diphosphate exchange, noncompetitive with respect to ATP
3'-O-Methyladenosine
-
ATP-diphosphate exchange, noncompetitive with respect to ATP
3-[(4-[[4-oxo-2-(phenylimino)-1,3-thiazolidin-5-ylidene]methyl]phenoxy) methyl]benzoic acid
-
3-[[([[2-(4-chlorophenyl)-4-quinolinyl]carbonyl]amino)carbothioyl]amino]benzoic acid
-
4,5,6,7-Tetrafluorotryptophan
-
-
5,7-Difluorotryptophan
-
-
5-hydroxytryptophan
-
ATP-diphosphate exchange, weak
5-Methyltryptophan
-
ATP-diphosphate exchange, weak
6-methyltryptophan
-
ATP-diphosphate exchange, weak
9-(2',3'-Dihydroxypropyl)adenine
-
S-isomer
9-(2'-Hydroxyethyl)adenine
-
-
9-(2'-Hydroxyethyl)adenine triphosphate
-
-
9-(3'-Hydroxypropyl)adenine
-
-
9-(3'-Hydroxypropyl)adenine triphosphate
-
-
9-(4'-Hydroxybutyl)adenine
-
-
9-(4'-Hydroxybutyl)adenine triphosphate
-
-
adenine
-
-
adenosine
-
-
Adenosine-5'-(beta-bromoethane phosphonate)
-
-
Adenosine-5'-(beta-bromoethanediphosphonate)
-
-
Adenosine-5'-(beta-chloroethyl phosphate)
-
-
Adenosine-5'-chloromethylphosphonate
-
ATP-diphosphate exchange, reversible, incompetitive
alpha,beta-methylene-ATP
-
-
beta-Indolyl propionic acid
-
-
beta-indolylacetic acid
-
-
beta-Indolylpyruvic acid
-
-
beta-Methyl-DL-tryptophan
-
ATP-diphosphate exchange
chuangximycin aldehyde
-
strong inhibition
chuangximycin lactone
-
-
chuangximycin nitrile
-
-
chuangxinmycin
D-tryptophan
-
-
dATP
-
-
diethyl dicarbonate
-
substrates protect, completely reactivated by hydroxylamine
DL-Tryptophanyl hydroxamate
-
ATP-diphosphate exchange
gamma-(n-Azidoanilide)-ATP
-
-
indolmycin
L-tryptophan
Mixed anhydride of ADP and mesithylenecarboxylic acid
-
-
-
Mixed anhydride of AMP and mesithylenecarboxylic acid
-
-
-
N-acetyl-L-tryptophan
-
-
N-Formyl-L-tryptophan
-
-
p-hydroxymercuribenzoate
-
-
succinylacetone
-
a heme biosynthesis inhibitor, inhibit cellular TrpRS activity in IFN-gamma-activated cells without affecting TrpRS protein expression
tryptamine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glyceraldehyde 3-phosphate dehydrogenase
-
the oxidized form of glyceraldehyde 3-phosphate dehydrogenase interacts with both full-length and mini tryptophanyl-tRNA synthetase and specifically stimulates the aminoacylation potential of mini, but not full-length tryptophanyl-tRNA synthetase. In contrast, reduced glyceraldehyde 3-phosphate dehydrogenase does not bind to tryptophanyl-tRNA synthetase and does not influence their aminoacylation activity
-
Hemin
-
heme can interact strongly with Zn2+-depleted full-length TrpRS, but not the Zn2+-bound enzyme, with a stoichiometric heme:protein ratio of 1:1 to enhance the aminoacylation activity significantly, involvement of heme in regulation of TrpRS aminoacylation activity, overview
interferon gamma
-
interferon regulatory factor 1
-
stimulates and regulates the enzyme isoforms expression via 2 specific interferon-gamma-sensitive promoters that are additionally regulated by the interferon gamma
-
interferons
-
interferons alpha, beta, and gamma, induction of accumulation of the enzyme, organelle-specific, the highest content is observed in the cytoplasm, followd by the nucleolus and the nucleus, the latter with a low enzyme content
-
serine hydroxamate
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00006
Archeoglobus fulgidus tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.00005
Archeoglobus fulgidus tRNATrp(A73C)
pH 7.0, 65°C
-
0.00006
Archeoglobus fulgidus tRNATrp(A73G)
pH 7.0, 65°C
-
0.00005
Archeoglobus fulgidus tRNATrp(A73U)
pH 7.0, 65°C
-
0.03 - 2
ATP
0.00009
Bacillus subtilis tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.00012
Bacillus subtilis tRNATrp(A73C)
pH 7.0, 65°C
-
0.0001
Bacillus subtilis tRNATrp(A73G)
pH 7.0, 65°C
-
0.00012
Bacillus subtilis tRNATrp(A73U)
pH 7.0, 65°C
-
0.00018
bovine tRNATrp(G73, wild-type)
pH 7.0, 65°C
-
0.00021
bovine tRNATrp(G73A)
pH 7.0, 65°C
-
0.00022
bovine tRNATrp(G73C)
pH 7.0, 65°C
-
0.00017
bovine tRNATrp(G73U)
pH 7.0, 65°C
-
0.00073 - 0.027
L-tryptophan
1
P1,P3-bis(5'-adenosyl)triphosphate
0.0021
tRNAArg
-
pH 7.5, 60°C
0.00012 - 0.0248
tRNATrp
0.0059
tRNATrp(A36C)
-
substrate mutant, pH 7.5, 60°C
-
0.0053
tRNATrp(A36G)
-
substrate mutant, pH 7.5, 60°C
-
0.0058
tRNATrp(A36U)
-
substrate mutant, pH 7.5, 60°C
-
0.0098
tRNATrp(A36U, A73G)
-
substrate mutant, pH 7.5, 60°C
-
0.0017
tRNATrp(A73C)
-
substrate mutant, pH 7.5, 60°C
-
0.0058
tRNATrp(A73G)
-
substrate mutant, pH 7.5, 60°C
-
0.0051
tRNATrp(A73U)
-
substrate mutant, pH 7.5, 60°C
-
0.06
tRNATrp(C34A)
-
substrate mutant, pH 7.5, 60°C
-
0.038
tRNATrp(C34G)
-
substrate mutant, pH 7.5, 60°C
-
0.048
tRNATrp(C34U)
-
substrate mutant, pH 7.5, 60°C
-
0.014
tRNATrp(C35A)
-
substrate mutant, pH 7.5, 60°C
-
0.038
tRNATrp(C35G)
-
substrate mutant, pH 7.5, 60°C
-
0.021
tRNATrp(C35U)
-
substrate mutant, pH 7.5, 60°C
-
0.025
tRNATrp(G1A-C72U)
-
substrate mutant, pH 7.5, 60°C
-
0.0055
tRNATrp(G1C-G72G)
-
substrate mutant, pH 7.5, 60°C
-
0.021
tRNATrp(G1U-C72A)
-
substrate mutant, pH 7.5, 60°C
-
0.015
tRNATrp(G2A-C71U)
-
substrate mutant, pH 7.5, 60°C
-
0.0019
tRNATrp(G2C-G71G)
-
substrate mutant, pH 7.5, 60°C
-
0.0058
tRNATrp(G2U-C71A)
-
substrate mutant, pH 7.5, 60°C
-
0.006
tRNATrp(HOG1)
-
substrate mutant, pH 7.5, 60°C
-
0.012
tRNATrp(pppG1)
-
substrate mutant, pH 7.5, 60°C
-
0.00045 - 0.128
Trp
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.7
ammonia
-
-
0.53
Archeoglobus fulgidus tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.11
Archeoglobus fulgidus tRNATrp(A73C)
pH 7.0, 65°C
-
0.07
Archeoglobus fulgidus tRNATrp(A73G)
pH 7.0, 65°C
-
0.09
Archeoglobus fulgidus tRNATrp(A73U)
pH 7.0, 65°C
-
1.1 - 419
ATP
0.14
Bacillus subtilis tRNATrp(A73, wild-type)
pH 7.0, 65°C
-
0.06
Bacillus subtilis tRNATrp(A73C)
pH 7.0, 65°C
-
0.04
Bacillus subtilis tRNATrp(A73G)
pH 7.0, 65°C
-
0.05
Bacillus subtilis tRNATrp(A73U)
pH 7.0, 65°C
-
0.03
bovine tRNATrp(G73, wild-type)
pH 7.0, 65°C
-
0.08
bovine tRNATrp(G73A)
pH 7.0, 65°C
-
0.06
bovine tRNATrp(G73C)
pH 7.0, 65°C
-
0.04
bovine tRNATrp(G73U)
pH 7.0, 65°C
-
0.59 - 24.5
L-tryptophan
0.16
tRNAArg
-
pH 7.5, 60°C
0.0009 - 16
tRNATrp
11
tRNATrp(A36C)
-
substrate mutant, pH 7.5, 60°C
-
4.4
tRNATrp(A36G)
-
substrate mutant, pH 7.5, 60°C
-
21
tRNATrp(A36U)
-
substrate mutant, pH 7.5, 60°C
-
0.27
tRNATrp(A36U, A73G)
-
substrate mutant, pH 7.5, 60°C
-
5.5
tRNATrp(A73C)
-
substrate mutant, pH 7.5, 60°C
-
0.62
tRNATrp(A73G)
-
substrate mutant, pH 7.5, 60°C
-
6.1
tRNATrp(A73U)
-
substrate mutant, pH 7.5, 60°C
-
0.17
tRNATrp(C34A)
-
substrate mutant, pH 7.5, 60°C
-
0.13
tRNATrp(C34G)
-
substrate mutant, pH 7.5, 60°C
-
0.11
tRNATrp(C34U)
-
substrate mutant, pH 7.5, 60°C
-
0.15
tRNATrp(C35A)
-
substrate mutant, pH 7.5, 60°C
-
0.079
tRNATrp(C35G)
-
substrate mutant, pH 7.5, 60°C
-
0.47
tRNATrp(C35U)
-
substrate mutant, pH 7.5, 60°C
-
2.3
tRNATrp(G1A-C72U)
-
substrate mutant, pH 7.5, 60°C
-
0.34
tRNATrp(G1C-G72G)
-
substrate mutant, pH 7.5, 60°C
-
0.46
tRNATrp(G1U-C72A)
-
substrate mutant, pH 7.5, 60°C
-
19
tRNATrp(G2A-C71U)
-
substrate mutant, pH 7.5, 60°C
-
1.7
tRNATrp(G2C-G71G)
-
substrate mutant, pH 7.5, 60°C
-
7.2
tRNATrp(G2U-C71A)
-
substrate mutant, pH 7.5, 60°C
-
3.2
tRNATrp(HOG1)
-
substrate mutant, pH 7.5, 60°C
-
4.5
tRNATrp(pppG1)
-
substrate mutant, pH 7.5, 60°C
-
2 - 419
Trp
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
164 - 1090
L-tryptophan
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0187 - 0.0228
2-[5-[(6-(ethoxycarbonyl)-5-(4-fluorophenyl)-3-oxo-7-phenyl-5H-[1,3]thiazolo[3,2-a]pyrimidin-2(3H)-ylidene)methyl]-2-furyl]benzoic acid
0.0151 - 0.0183
3-[(4-[[4-oxo-2-(phenylimino)-1,3-thiazolidin-5-ylidene]methyl]phenoxy) methyl]benzoic acid
0.0352 - 0.0422
3-[[([[2-(4-chlorophenyl)-4-quinolinyl]carbonyl]amino)carbothioyl]amino]benzoic acid
0.000002
indolmycin
Geobacillus stearothermophilus;
-
pH 8.0, 37°C, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0018
-
purified enzyme
0.45
-
-
1.45
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
in cacodylate buffer
7.8
-
assay at
8.5
-
in Hepes buffer
8.5 - 9.2
-
in Tris buffer
9
exchange assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
tryptophanylation assay
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
peripheral blood mononuclear cells
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
monocyte-derived mature
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
high expression
Manually annotated by BRENDA team
-
umbilical vein
Manually annotated by BRENDA team
-
monocyte
Manually annotated by BRENDA team
-
enzyme TrpRS is overexpressed in OSCC tissues (139/146, 95.2%) compared with adjacent normal tissues. TrpRS knockdown or treatment with conditioned media obtained from TrpRS-knockdown cells significantly reduce oral cancer cell viability and invasiveness. TrpRS overexpression promotes cell migration and invasion. In addition, the extracellular addition of TrpRS rescues the invasion ability of TrpRS-knockdown cells. No significant association between TrpRS level and gender, age or N stage
Manually annotated by BRENDA team
-
CD4+ cell, CD8+ cell
Manually annotated by BRENDA team
embryonic, developing
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
about 0.2% of total soluble protein
Manually annotated by BRENDA team
-
endothelial cell
Manually annotated by BRENDA team
caruncular endometrial tissues of unilaterally pregnant ewes at implantation (day 16) and post-implantation (day 20) periods. WARS protein expression increased in caruncular tissues of the gravid uterine horns compared with the non-gravid uterine horns on days 16 and 20 of pregnancy. On day 16 of pregnancy, there are no differences in WARS mRNA expression between the gravid and non-gravid uterine horns but expression of the gene is higher in the gravid uterine horns when compared with the non-gravid uterine horns on day 20 of pregnancy
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
low content
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
UNIPROT
ORGANISM
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1);
P21656
Bacillus subtilis (strain 168);
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168);
Q5CYP8
Cryptosporidium parvum (strain Iowa II);
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422);
A0A077EER2
Elizabethkingia anophelis NUHP1;
O96771
Encephalitozoon cuniculi (strain GB-M1);
P67589
Escherichia coli O157:H7;
Geobacillus stearothermophilus;
A8B8N3
Giardia intestinalis (strain ATCC 50803 / WB clone C6);
P43835
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd);
Homo sapiens;
P75510
Mycoplasma pneumoniae (strain ATCC 29342 / M129);