EC Number |
Protein Variants |
Reference |
---|
4.2.2.7 | K185A |
about 100% of wild-type activity |
-, 704591 |
4.2.2.7 | Q22A |
about 20% of wild-type activity |
-, 704591 |
4.2.2.7 | K252A |
about 40% of wild-type activity |
704591 |
4.2.2.7 | Q223A |
about 40% of wild-type activity |
704591 |
4.2.2.7 | DELTA 191-213 |
about 5% of wild-type activity |
704591 |
4.2.2.7 | R156A |
about 90% of wild-type activity |
-, 704591 |
4.2.2.7 | more |
construction of several double and triple mutants of the calcium-coordinating residues |
651971 |
4.2.2.7 | H202/H406A |
His-tagged version expressed in Escherichia coli BL21(DE3) |
704673 |
4.2.2.7 | H202A/Y257A |
His-tagged version expressed in Escherichia coli BL21(DE3) |
704673 |
4.2.2.7 | Y257A/H406A |
His-tagged version expressed in Escherichia coli BL21(DE3) |
704673 |