EC Number   |
Protein Variants |
Reference |
|---|
    4.2.1.125 | more |
establishment of dammarane-type ginsenosides synthesis from 2,3-oxidosqualene in Oryza sativa by expression of dammarenediol-II synthase from Panax ginseng resulting in a dammarane-type sapogenin 20(S)-protopanaxadiol (PPD) content of 0.35-0.59 mg/gdw and a dammarane-type sapogenin 20(S)-protopanaxatriol (PPT) content of 0.23-0.43 mg/g dry weight in the transgenic rice plants |
749016 |
| 4.2.1.125 | more |
expression in Saccharomyces cerevisiae mutant lacking lanosterol synthase results in production of dammarenediol and hydroxydammarenone. Silencing of gene expression by RNAi leads to a reduction of ginsenoide production to 84.5% in roots |
683979 |
| 4.2.1.125 | more |
functionally expressed in the lanosterol synthase-deficient yeast mutant erg7 |
710295 |
| 4.2.1.125 | more |
heterologous biosynthesis of triterpenoid dammarenediol-II, the precursor of dammarane-type tetracyclic ginsenosides, in engineered Escherichia coli strain BL21(DE3) by expressing the enzyme, and coexpressing squalene synthase, squalene epoxidase, and NADPH-cytochrome P450 reductase from Saccharomyces cerevisiae, and squalene epoxidase from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana, and thereby reconstituting the 2,3-oxidosqualene-derived triterpenoid biosynthetic pathway. Method evaluation and optimization, overview |
747390 |
| 4.2.1.125 | more |
identification of single nucleotide polymorphisms in different samples of Panax ginseng cultivar DAMAYA, specific single nucleotide polymorphisms (SNPs) of the DDS gene might be associated with the accumulation of some ginsenosides in DAMAYA ginseng and thus can be used for varietal characterization, molecular identification, qualitative evaluation of ginseng-type medicinal materials, or gene engineering and ginseng breeding. The ginsenoside contents, of such as Rf, Rg1 and F1, change with the gene mutation |
748874 |
| 4.2.1.125 | more |
metabolic engineering of Pichia pastoris strain GS115 for the high yield production of dammarenediol-II, based on the native triterpene synthetic pathway, a dammarenediol-II synthetic pathway is established in Pichia pastoris by introducing a dammarenediol-II synthase gene (PgDDS) from Panax ginseng. To increase the supply of 2,3-oxidosqualene, the ERG1 gene, which is responsible for 2,3-oxidosqualene synthesis, is coexpressed. And to reduce competition for 2,3-oxidosqualene from ergosterol biosynthesis without affecting the normal growth of Pichia pastoris, the ERG7gene, which is responsible for conversion of 2,3-oxidosqualene to lanosterol, is downregulated by replacing its native promoter with a thiamine-repressible promoter, using a marker-recycling and gene-targeting Cre- lox71/66 system, developed for Pichia pastoris. Method evaluation and optimization, overview |
748268 |
| 4.2.1.125 | C264A |
site-directed mutagenesis, the mutant shows 53% decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | C489A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | D488A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
| 4.2.1.125 | F477A |
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme |
748118 |
