Literature summary for 4.2.1.125 extracted from

Li, D.; Zhang, Q.; Zhou, Z.; Zhao, F.; Lu, W.
Heterologous biosynthesis of triterpenoid dammarenediol-II in engineered Escherichia coli (2016), Biotechnol. Lett., 38, 603-609 .

Search for more literature for 4.2.1.125

Cloned(Commentary)

Cloned (Comment)	Organism
gene PNA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of squalene synthase (UniProt ID P29704), squalene epoxidase (UniProt ID P32476), and NADPH-cytochrome P450 reductase (UniProt ID P16603) from Saccharomyces cerevisiae, and squalene epoxidase (UniProt ID Q603D5) from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana (UniProt ID Q9SB48)	Panax ginseng

Cloned (Comment)

Organism

gene PNA, recombinant expression in Escherichia coli strain BL21(DE3), coexpression of squalene synthase (UniProt ID P29704), squalene epoxidase (UniProt ID P32476), and NADPH-cytochrome P450 reductase (UniProt ID P16603) from Saccharomyces cerevisiae, and squalene epoxidase (UniProt ID Q603D5) from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana (UniProt ID Q9SB48)

Panax ginseng

Protein Variants

Protein Variants	Comment	Organism
additional information	heterologous biosynthesis of triterpenoid dammarenediol-II, the precursor of dammarane-type tetracyclic ginsenosides, in engineered Escherichia coli strain BL21(DE3) by expressing the enzyme, and coexpressing squalene synthase, squalene epoxidase, and NADPH-cytochrome P450 reductase from Saccharomyces cerevisiae, and squalene epoxidase from Methylococcus capsulatus, as well as NADPH-cytochrome P450 reductase from Arabidopsis thaliana, and thereby reconstituting the 2,3-oxidosqualene-derived triterpenoid biosynthetic pathway. Method evaluation and optimization, overview	Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O	Panax ginseng	-	dammarenediol II	-	r

Organism

Organism	UniProt	Comment	Textmining
Panax ginseng	Q08IT1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(3S)-2,3-epoxy-2,3-dihydrosqualene + H2O	-	Panax ginseng	dammarenediol II	-	r

Synonyms

Synonyms	Comment	Organism
dammarenediol-II synthase	-	Panax ginseng
DDS	-	Panax ginseng
PNA	-	Panax ginseng

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	-	Panax ginseng

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Panax ginseng