EC Number   |
Protein Variants |
Reference |
|---|
   4.1.2.10 | D208N |
less than 2% residual activity |
727309 |
| 4.1.2.10 | E54A |
completely inactive mutant enzyme |
747750 |
| 4.1.2.10 | E54Q |
mutant enzyme exhibits about 8 of wild-type activity |
747750 |
| 4.1.2.10 | H236F |
less than 2% residual activity |
727309 |
| 4.1.2.10 | H459N |
less than 5% of the activity compared to wild type |
702292 |
| 4.1.2.10 | H497N |
less than 5% of the activity compared to wild type |
702292 |
| 4.1.2.10 | H8A |
completely inactive mutant enzyme |
747750 |
| 4.1.2.10 | I108M/A111G |
mutant with improved catalytic properties: recovery of a 89.9% yield of (R)-2-chloromandelonitrile with an enantiomeric excess of 97.6% enantiomeric excess, about 4% residual aldehyde. The mutant also contains two silent mutations at position 85 and position 432 |
691796 |
| 4.1.2.10 | L1Q |
mutation enhances expression in Pichia pastoris |
691796 |
| 4.1.2.10 | L1Q/A111G |
activity is similar to wild-type enzyme with its preferred substrate benzaldehyde. Directed evolution of PaHNL5/L1Q/A11G |
691796 |
