Literature summary for 4.1.2.10 extracted from

Andexer, J.N.; Staunig, N.; Eggert, T.; Kratky, C.; Pohl, M.; Gruber, K.
Hydroxynitrile lyases with alpha/beta-hydrolase fold: two enzymes with almost identical 3D structures but opposite enantioselectivities and different reaction mechanisms (2012), ChemBioChem, 13, 1932-1939.

Cloned(Commentary)

Cloned (Comment)	Organism
-	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
structure at a crystallographic resolution of 2.5 A and comparison with the S-selective HNL from Hevea brasiliensis. The structures exhibit an alpha/beta-hydrolase fold and a Ser-His-Asp catalytic triad. Modeling of complexes of the enzymes with both (R)- and (S)-mandelonitrile leads to a catalytic mechanism, in which His236 from the catalytic triad acts as a general base and the emerging negative charge on the cyano group is stabilized by main-chain amide groups and an alpha-helix dipole very similar to alpha/beta-hydrolases	Arabidopsis thaliana

Crystallization (Comment)

Organism

structure at a crystallographic resolution of 2.5 A and comparison with the S-selective HNL from Hevea brasiliensis. The structures exhibit an alpha/beta-hydrolase fold and a Ser-His-Asp catalytic triad. Modeling of complexes of the enzymes with both (R)- and (S)-mandelonitrile leads to a catalytic mechanism, in which His236 from the catalytic triad acts as a general base and the emerging negative charge on the cyano group is stabilized by main-chain amide groups and an alpha-helix dipole very similar to alpha/beta-hydrolases

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
D208N	less than 2% residual activity	Arabidopsis thaliana
H236F	less than 2% residual activity	Arabidopsis thaliana
M237K	no residual activity	Arabidopsis thaliana
M237L	100% residual activity	Arabidopsis thaliana
N12T	less than 2% residual activity	Arabidopsis thaliana
N12T/M237K	expression results in insoluble protein	Arabidopsis thaliana
S81A	less than 2% residual activity	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LFT6	-	-

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Release 2023.1 (January 2023)