EC Number   |
Protein Variants |
Reference |
|---|
    3.5.4.16 | C110G |
site-directed mutagenesis, 0.22% activity compared to the wild-type enzyme, increased temperature optimum |
655934 |
| 3.5.4.16 | C110S |
highly reduced activity in both reaction steps using GTP or 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone triphosphate as substrate, reduced zinc content compared to the wild-type enzyme |
656511 |
| 3.5.4.16 | C149A |
mutant enzyme shows no activity |
-, 756001 |
| 3.5.4.16 | C149A |
mutant protein purified under the same conditions as wild-type enzyme lacks any S-nitrosylated thiols. S-nitrosylation of the wild-type enzyme occurs posttranslationally during heterologous expression in Escherichia coli and that it is specific to Cys149 |
-, 756001 |
| 3.5.4.16 | C149S |
mutant enzyme exhibits 2.7% of the wild-type activity |
-, 756001 |
| 3.5.4.16 | C181S |
determination of crystal structure, highly reduced activity in both reaction steps using GTP or 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone triphosphate as substrate, reduced zinc content compared to the wild-type enzyme |
656511 |
| 3.5.4.16 | C181S |
site-directed mutagenesis, 0.29% activity compared to the wild-type enzyme, highly increased temperature optimum |
655934 |
| 3.5.4.16 | C265T |
mutation results in the loss of the 177 C-terminal amino acids |
688532 |
| 3.5.4.16 | E111K |
site-directed mutagenesis, 3.1% activity compared to the wild-type enzyme, increased temperature optimum |
655934 |
| 3.5.4.16 | E152A |
mutant enzyme exhibits about 20% of the wild-type activity |
756001 |
