Literature summary for 3.5.4.16 extracted from

Paranagama, N.; Bonnett, S.A.; Alvarez, J.; Luthra, A.; Stec, B.; Gustafson, A.; Iwata-Reuyl, D.; Swairjo, M.A.
Mechanism and catalytic strategy of the prokaryotic-specific GTP cyclohydrolase-IB (2017), Biochem. J., 474, 1017-1039 .

Search for more literature for 3.5.4.16

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed as an N-terminally His6 tagged protein in Bl21(DE3) cells	Neisseria gonorrhoeae

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are grown at 20°C by vapor diffusion in sitting drops. High-resolution crystal structures of the enzyme: one in complex with the reaction intermediate analog and competitive inhibitor 8-oxo-GTP, and one with a TRIS molecule bound in the active site and mimicking another reaction intermediate	Neisseria gonorrhoeae

Protein Variants

Protein Variants	Comment	Organism
C149A	mutant enzyme shows no activity	Neisseria gonorrhoeae
C149A	mutant protein purified under the same conditions as wild-type enzyme lacks any S-nitrosylated thiols. S-nitrosylation of the wild-type enzyme occurs posttranslationally during heterologous expression in Escherichia coli and that it is specific to Cys149	Neisseria gonorrhoeae
C149S	mutant enzyme exhibits 2.7% of the wild-type activity	Neisseria gonorrhoeae
E152A	mutant enzyme exhibits about 20% of the wild-type activity	Neisseria gonorrhoeae
E243A	mutant enzyme exhibits 0.37% of the wild-type activity	Neisseria gonorrhoeae
H246A	mutant enzyme exhibits 0.74% of the wild-type activity	Neisseria gonorrhoeae
H246D	mutant enzyme exhibits 3.0% of the wild-type activity	Neisseria gonorrhoeae
H246K	mutant enzyme shows no activity	Neisseria gonorrhoeae
H246N	mutant enzyme shows no activity	Neisseria gonorrhoeae
H246Q	mutant enzyme exhibits 2.4% of the wild-type activity	Neisseria gonorrhoeae

Inhibitors

Inhibitors	Comment	Organism	Structure
2'-deoxy-GTP	-	Neisseria gonorrhoeae
7-deaza-GTP	-	Neisseria gonorrhoeae
8-oxo-GTP	competitive inhibitor. The inhibitor interacts with a conserved active site Cys149, and this residue is S-nitrosylated. Ki/Km: 0.022	Neisseria gonorrhoeae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00915	-	GTP	pH and temperature not specified in the publication	Neisseria gonorrhoeae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	Zn2+-dependent enzyme	Neisseria gonorrhoeae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
135000	-	gel filtration	Neisseria gonorrhoeae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP + H2O	Neisseria gonorrhoeae	-	formate + 7,8-dihydroneopterin 3'-triphosphate	-	?
GTP + H2O	Neisseria gonorrhoeae ATCC 700825	-	formate + 7,8-dihydroneopterin 3'-triphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Neisseria gonorrhoeae	Q5F9K6	-	-
Neisseria gonorrhoeae ATCC 700825	Q5F9K6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Neisseria gonorrhoeae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Neisseria gonorrhoeae	formate + 7,8-dihydroneopterin 3'-triphosphate	-	?
GTP + H2O	-	Neisseria gonorrhoeae ATCC 700825	formate + 7,8-dihydroneopterin 3'-triphosphate	-	?

Subunits

Subunits	Comment	Organism
homotetramer	-	Neisseria gonorrhoeae

Synonyms

Synonyms	Comment	Organism
FelE	-	Neisseria gonorrhoeae
GCYH-IB	-	Neisseria gonorrhoeae
GTP cyclohydrolase IB	-	Neisseria gonorrhoeae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000265	-	GTP	pH and temperature not specified in the publication	Neisseria gonorrhoeae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000149	-	8-oxo-GTP	pH and temperature not specified in the publication	Neisseria gonorrhoeae
0.0457	-	7-deaza-GTP	pH and temperature not specified in the publication	Neisseria gonorrhoeae
9.164	-	2'-deoxy-GTP	pH and temperature not specified in the publication	Neisseria gonorrhoeae

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Release 2023.1 (January 2023)