EC Number   |
Protein Variants |
Reference |
|---|
   3.1.3.46 | A44G |
site directed mutagenesis |
681437 |
| 3.1.3.46 | A44V |
site directed mutagenesis |
681437 |
| 3.1.3.46 | E325A |
role of E325 as acid-base during reaction |
649951 |
| 3.1.3.46 | E327A |
catalytic site mutation, role in maintaining the structural integrity |
650014 |
| 3.1.3.46 | E327A |
site-directed mutagenesis, inactive mutant, can be rescued by formate at pH 7.0, overview |
663563 |
| 3.1.3.46 | H258A |
site-directed mutagenesis, mutation of the strong nucleophile His258 reduces activity compared to the wild-type enzyme |
663563 |
| 3.1.3.46 | H444A/R445A/R447A |
enhances catalytic rate of bisphosphatase activity and abrogates inhibition by kinase domain |
640339 |
| 3.1.3.46 | H446A |
site-directed mutagenesis, the mutation perturbs significantly and specificially the resonances at the catalytic site assigned to His258 and His392 in His-specific multinuclear NMR study, interaction of residue 446 with R397 is disrupted, comparison to the wild-type enzyme, overview |
663563 |
| 3.1.3.46 | K168A |
site directed mutagenesis |
681437 |
| 3.1.3.46 | K168N |
site directed mutagenesis |
681437 |
