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Literature summary for 3.1.3.46 extracted from
- A direct substrate-substrate interaction found in the kinase domain of the bifunctional enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (2007), J. Mol. Biol., 370, 14-26.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli as His6-tagged fusion protein | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| by the sitting-drop, vapor-diffusion method, crystals of PFKFB D-fructose 6-phosphate complex in the presence of the nonreactive ATP-analogue AMPPCP beta,gamma-methyleneadenosine 5-triphosphate and crystals the inhibitory complex of PFKFB ADP and phosphoenolpyruvate | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A44G | site directed mutagenesis | Homo sapiens |
| A44V | site directed mutagenesis | Homo sapiens |
| K168A | site directed mutagenesis | Homo sapiens |
| K168N | site directed mutagenesis | Homo sapiens |
| K168R | site directed mutagenesis | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphoenolpyruvate | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0102 | - |
beta-D-fructose 2,6-bisphosphate | wild type enzyme | Homo sapiens | |
| 0.038 | - |
beta-D-fructose 2,6-bisphosphate | A144G mutant | Homo sapiens | |
| 0.204 | - |
beta-D-fructose 2,6-bisphosphate | A144V mutant | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA affinity columns | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate | in-line phosphoryl transfer initiated by a substrate-assisted mechanism | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-fructose 2,6-bisphosphate + H2O | - |
Homo sapiens | D-fructose 6-phosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-phosphofructo-2-kinase/fructose-2 6-biphosphatase | - |
Homo sapiens |
| PFKFB3 | - |
Homo sapiens |
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