EC Number   |
Protein Variants |
Reference |
|---|
   2.8.2.38 | G301D |
exchange of amino acid from ecotype C24 to that of ecotype Col0. 25fold increase in specific activity with desulfobenzylglucosinolate |
742479 |
| 2.8.2.38 | H155A |
site-directed mutagenesis, inactive mutant |
-, 762438 |
| 2.8.2.38 | K93A |
site-directed mutagenesis, inactive mutant |
-, 762438 |
| 2.8.2.38 | more |
generation of two chimeric cytochrome P450 enzymes from CYP79A2 and CYP83B1 are constructed and functionally expressed in Escherichia coli. The Escherichia coli cystathionine beta-lyase is used to replace the plant-derived CS lyase whose active form cannot be expressed in Escherichia coli strain BL21(DE3). Reconstruction of the BITC biosynthesis pathway from plants in the bacteria, involving CYP79A2 and CYP83B1, and the glutathione S-transferase, gamma-glutamyl peptidase, C-S lyase, glucosyltransferase, 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase, and myrosinase are encoded by genes GSTF, GGP1, SUR1, UGT74B1, SOT18, and MYR, respectively, overview |
-, 760279 |
| 2.8.2.38 | N339K |
exchange of amino acid from ecotype C24 to that of ecotype Col0, residue glycine at position 301 may play a critical role in substrate recognition |
742479 |
| 2.8.2.38 | P136A |
site-directed mutagenesis, the mutant shows unaltered activity compared to wild-type enzyme |
762438 |
| 2.8.2.38 | T96A |
site-directed mutagenesis, mutant Thr96Ala shows residual activity with the preferred substrates 3-methylthiopropyl desulfoglucosinolate and 8-methylthiooctyl desulfoglucosinolate (12fold reduction and 3fold reduction, respectively), while with sinigrin no activity is detected |
-, 762438 |
| 2.8.2.38 | T97A |
site-directed mutagenesis, inactive mutant |
-, 762438 |
| 2.8.2.38 | Y130A |
site-directed mutagenesis, inactive mutant |
-, 762438 |
