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3'-phosphoadenylyl sulfate + 3-methylthiopropyl desulfoglucosinolate
adenosine 3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-2-phenylethyl glucosinolate
adenosine 3',5'-bisphosphate + 2-phenylethyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-butenylglucosinolate
adenosine 3',5'-bisphosphate + 3-butenylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropyl glucosinolate
adenosine-3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropylglucosinolate
adenosine 3',5'-bisphosphate + 3-methylthiopropylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutyl glucosinolate
adenosine-3',5'-bisphosphate + 4-methylthiobutyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
3'-phosphoadenylyl sulfate + desulfo-5-methylthiopentylglucosinolate
adenosine 3',5'-bisphosphate + 5-methylthiopentylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-6-methylthiohexylglucosinolate
adenosine 3',5'-bisphosphate + 6-methylthiohexylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptylglucosinolate
adenosine 3',5'-bisphosphate + 7-methylthioheptylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctylglucosinolate
adenosine 3',5'-bisphosphate + 8-methylthiooctylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-benzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-indole-3-methylglucosinolate
adenosine 3',5'-bisphosphate + indole-3-methylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfosinigrin
adenosine 3',5'-bisphosphate + sinigrin
adenosine 3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
3'-phosphoadenylyl sulfate + 7-methylthioheptyl desulfoglucosinolate
adenosine 3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
3'-phosphoadenylyl sulfate + 8-methylthiooctyl desulfoglucosinolate
adenosine 3',5'-bisphosphate + glucoerucin
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
adenosine 3',5'-bisphosphate + sinigrin
3'-phosphoadenylyl sulfate + desulfosinigrin
sinigrin binding is facilitated by hydrogen bonding with residues Arg51, Glu54, Thr96, Tyr130, His155 and Tyr306. The guanidinium group of Arg51 interacts with the 6'-hydroxyl group of the glucopyranose of the glucosinolate. The carboxyl group of Glu54 interacts with the 4'- and the 6'-hydroxyl groups. The hydroxyl of Tyr306 forms a hydrogen bond with the oxygen in the glucopyranose ring. The sulfate moiety is stabilized by hydrogen bonds with Thr96, Tyr130 and His155. In this positioning the sulfate and phosphate groups of sinigrin and PAP, respectively, are located within the hydrogen bond distance from each other. Analysis of the mechanism of PAPS binding
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-
?
additional information
?
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3'-phosphoadenylyl sulfate + 3-methylthiopropyl desulfoglucosinolate

adenosine 3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + 3-methylthiopropyl desulfoglucosinolate
adenosine 3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate

adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate

adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
preferred substrate of isozyme SOT18 of ecotype C24
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-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate

adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
-
-
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
preferred substrate of isozyme SOT18 of ecotype C24
-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate

adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
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-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
preferred substrate of isozyme SOT16 of ecotype C24
-
-
?
3'-phosphoadenylyl sulfate + desulfosinigrin

adenosine 3',5'-bisphosphate + sinigrin
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-
?
3'-phosphoadenylyl sulfate + desulfosinigrin
adenosine 3',5'-bisphosphate + sinigrin
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-
-
?
adenosine 3',5'-bisphosphate + 7-methylthioheptyl glucosinolate

3'-phosphoadenylyl sulfate + 7-methylthioheptyl desulfoglucosinolate
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-
-
?
adenosine 3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
3'-phosphoadenylyl sulfate + 7-methylthioheptyl desulfoglucosinolate
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-
?
adenosine 3',5'-bisphosphate + 8-methylthiooctyl glucosinolate

3'-phosphoadenylyl sulfate + 8-methylthiooctyl desulfoglucosinolate
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-
-
?
adenosine 3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
3'-phosphoadenylyl sulfate + 8-methylthiooctyl desulfoglucosinolate
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-
?
adenosine 3',5'-bisphosphate + glucoerucin

?
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-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
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?
adenosine 3',5'-bisphosphate + glucoerucin
?
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?
adenosine 3',5'-bisphosphate + glucoerucin
?
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?
adenosine 3',5'-bisphosphate + glucoerucin
?
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?
adenosine 3',5'-bisphosphate + glucoerucin
?
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-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
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?
adenosine 3',5'-bisphosphate + glucoiberverin

?
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-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
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-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
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?
adenosine 3',5'-bisphosphate + glucoiberverin
?
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?
adenosine 3',5'-bisphosphate + glucoiberverin
?
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?
adenosine 3',5'-bisphosphate + glucoiberverin
?
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?
adenosine 3',5'-bisphosphate + glucoiberverin
?
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?
additional information

?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
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?
additional information
?
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qualitative glucosinolate analysis in leaves of ecotype C24, overview
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?
additional information
?
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qualitative glucosinolate analysis in leaves of ecotype C24, overview
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?
additional information
?
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qualitative glucosinolate analysis in leaves of ecotype C24, overview
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?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
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?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
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-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
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-
?
additional information
?
-
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
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-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
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?
additional information
?
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ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
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?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
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-
?
additional information
?
-
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ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
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-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
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-
?
additional information
?
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ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
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?
additional information
?
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ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
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?
additional information
?
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ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
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?
additional information
?
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enzyme AtSOT18 prefers the long-chain ds-Gls 7-methylthioheptyl Gl and 8-methylthiooctyl Gl (8MTO), that are also derived from methionine
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additional information
?
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enzyme AtSOT18 prefers the long-chain ds-Gls 7-methylthioheptyl Gl and 8-methylthiooctyl Gl (8MTO), that are also derived from methionine
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additional information
?
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usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies
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additional information
?
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usage of 3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase from Arabidopsis thaliana ecotype Col-0 and myrosinase from Brevicoryne brassicae for the biosynthesis of benzyl isothiocyanate by the combined expression of the optimized enzymes in vitro, product identity is confirmed by gas chromatography/mass spectrometry analysis. SOTs from different Arabidopsis ecotypes show different efficiencies
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additional information
?
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enzyme AtSOT18 prefers the long-chain ds-Gls 7-methylthioheptyl Gl and 8-methylthiooctyl Gl (8MTO), that are also derived from methionine
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additional information
?
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enzyme prefers long-chained methionine-derived desulfoglucosinolates desulfo4-methythiobutylglucosinolate and desulfo-5-methylthiopentylglucosinoate
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?
additional information
?
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enzyme prefers long-chained methionine-derived desulfoglucosinolates desulfo4-methythiobutylglucosinolate and desulfo-5-methylthiopentylglucosinoate
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?
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0.06 - 0.25
3'-phosphoadenylyl sulfate
0.055 - 0.271
desulfo-3-methylthiopropylglucosinolate
0.043 - 0.13
desulfo-4-methylthiobutylglucosinolate
0.05 - 0.1
desulfobenzylglucosinolate
0.055 - 0.1
glucoiberverin
additional information
additional information
-
0.06
3'-phosphoadenylyl sulfate

pH 9.0, 30°C
0.25
3'-phosphoadenylyl sulfate
pH 9.0, 30°C
0.055
desulfo-3-methylthiopropylglucosinolate

pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0
0.07
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT16
0.088
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT17
0.1
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype C24
0.271
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C
0.043
desulfo-4-methylthiobutylglucosinolate

pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0
0.065
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT17
0.08
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT16
0.09
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C
0.13
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype C24
0.05
desulfobenzylglucosinolate

pH 9.0, 30°C
0.1
desulfobenzylglucosinolate
pH 9.0, 30°C
0.043
glucoerucin

-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.058
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.06
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.081
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.096
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.13
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.055
glucoiberverin

-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.06
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.067
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.09
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.1
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
additional information
additional information

kinetics
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additional information
additional information
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kinetics
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additional information
additional information
kinetics, overview
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additional information
additional information
kinetics, overview
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additional information
additional information
kinetics, overview
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additional information
additional information
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kinetics, overview
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