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3'-phosphoadenylyl sulfate + desulfo-2-phenylethyl glucosinolate
adenosine 3',5'-bisphosphate + 2-phenylethyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-butenylglucosinolate
adenosine 3',5'-bisphosphate + 3-butenylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropyl glucosinolate
adenosine-3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropylglucosinolate
adenosine 3',5'-bisphosphate + 3-methylthioproylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutyl glucosinolate
adenosine-3',5'-bisphosphate + 4-methylthiobutyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
3'-phosphoadenylyl sulfate + desulfo-5-methylthiopentylglucosinolate
adenosine 3',5'-bisphosphate + 5-methylthiopentylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-6-methylthiohexylglucosinolate
adenosine 3',5'-bisphosphate + 6-methylthiohexylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptylglucosinolate
adenosine 3',5'-bisphosphate + 7-methylthioheptylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctylglucosinolate
adenosine 3',5'-bisphosphate + 8-methylthiooctylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-benzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-indole-3-methylglucosinolate
adenosine 3',5'-bisphosphate + indole-3-methylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
additional information
?
-
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate

adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate

adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
preferred substrate of isozyme SOT18 of ecotype C24
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate

adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
preferred substrate of isozyme SOT18 of ecotype C24
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate

adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
preferred substrate of isozyme SOT16 of ecotype C24
-
?
adenosine 3',5'-bisphosphate + glucoerucin

?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoerucin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin

?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
additional information

?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
enzyme prefers long-chained methionine-derived desulfoglucosinolates desulfo4-methythiobutylglucosinolate and desulfo-5-methylthiopentylglucosinoate
-
?
additional information
?
-
-
enzyme prefers long-chained methionine-derived desulfoglucosinolates desulfo4-methythiobutylglucosinolate and desulfo-5-methylthiopentylglucosinoate
-
?
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0.06 - 0.25
3'-phosphoadenylyl sulfate
0.055 - 0.271
desulfo-3-methylthiopropylglucosinolate
0.043 - 0.13
desulfo-4-methylthiobutylglucosinolate
0.05 - 0.1
desulfobenzylglucosinolate
0.055 - 0.1
glucoiberverin
additional information
additional information
-
0.06
3'-phosphoadenylyl sulfate

pH 9.0, 30°C
0.25
3'-phosphoadenylyl sulfate
pH 9.0, 30°C
0.055
desulfo-3-methylthiopropylglucosinolate

pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0
0.07
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT16
0.088
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT17
0.1
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype C24
0.271
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C
0.043
desulfo-4-methylthiobutylglucosinolate

pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0
0.065
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT17
0.08
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT16
0.09
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C
0.13
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype C24
0.05
desulfobenzylglucosinolate

pH 9.0, 30°C
0.1
desulfobenzylglucosinolate
pH 9.0, 30°C
0.043
glucoerucin

-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.058
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.06
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.081
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.096
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.13
glucoerucin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.055
glucoiberverin

-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.06
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.067
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.09
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
0.1
glucoiberverin
-
in 80 mM Tris/HCl, pH 9.0, 9.2 mM MgCl2, at 37°C
additional information
additional information

kinetics, overview
-
additional information
additional information
kinetics, overview
-
additional information
additional information
kinetics, overview
-
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Klein, M.; Papenbrock, J.
Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana
Physiol. Plant.
135
140-149
2009
Arabidopsis thaliana (Q9C9C9), Arabidopsis thaliana (Q9C9D0), Arabidopsis thaliana (Q9FZ80)
brenda
Luczak, S.; Forlani, F.; Papenbrock, J.
Desulfo-glucosinolate sulfotransferases isolated from several Arabidopsis thaliana ecotypes differ in their sequence and enzyme kinetics
Plant Physiol. Biochem.
63
15-23
2013
Arabidopsis thaliana, Arabidopsis thaliana ecotype C24, Arabidopsis thaliana ecotype Cvi-0, Arabidopsis thaliana ecotype Col-0, Arabidopsis thaliana ecotype Ws, Arabidopsis thaliana ecotype Bla-10, Arabidopsis thaliana ecotype Ler-1
brenda
Klein, M.; Reichelt, M.; Gershenzon, J.; Papenbrock, J.
The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed
FEBS J.
273
122-136
2006
Arabidopsis thaliana (Q9C9C9), Arabidopsis thaliana (Q9FZ80)
-
brenda
Piotrowski, M.; Schemenewitz, A.; Lopukhina, A.; Müller, A.; Janowitz, T.; Weiler, E.; Oecking, C.
Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure
J. Biol. Chem.
279
50717-50725
2004
Arabidopsis thaliana (Q9C9C9), Arabidopsis thaliana (Q9FZ80)
brenda
Hirschmann, F.; Papenbrock, J.
The fusion of genomes leads to more options A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana
Plant Physiol. Biochem.
91
10-19
2015
Brassica napus (A0A0K1H0V2), Brassica napus
brenda