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Information on EC 2.8.2.38 - aliphatic desulfoglucosinolate sulfotransferase
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2.8.2.38 aliphatic desulfoglucosinolate sulfotransferaseIUBMB Comments
The enzyme catalyses the last step in the biosynthesis of aliphatic glucosinolate core structures. cf. EC 2.8.2.24, aromatic desulfoglucosinolate sulfotransferase.
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
sot18, ds-gl sot, sot16, sot17, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
desulfo-glucosinolate sulfotransferase
SOT17
SOT18
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3'-phosphoadenylyl sulfate + an aliphatic desulfoglucosinolate = adenosine 3',5'-bisphosphate + an aliphatic glucosinolate
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
glucosinolate biosynthesis from dihomomethionine, glucosinolate biosynthesis from hexahomomethionine, glucosinolate biosynthesis from homomethionine, glucosinolate biosynthesis from pentahomomethionine, glucosinolate biosynthesis from tetrahomomethionine, glucosinolate biosynthesis from trihomomethionine
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SYSTEMATIC NAME
IUBMB Comments
3'-phosphoadenylyl-sulfate:aliphatic desulfoglucosinolate sulfotransferase
The enzyme catalyses the last step in the biosynthesis of aliphatic glucosinolate core structures. cf. EC 2.8.2.24, aromatic desulfoglucosinolate sulfotransferase.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3'-phosphoadenylyl sulfate + desulfo-2-phenylethyl glucosinolate
adenosine 3',5'-bisphosphate + 2-phenylethyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-butenylglucosinolate
adenosine 3',5'-bisphosphate + 3-butenylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropyl glucosinolate
adenosine-3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropylglucosinolate
adenosine 3',5'-bisphosphate + 3-methylthioproylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutyl glucosinolate
adenosine-3',5'-bisphosphate + 4-methylthiobutyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
3'-phosphoadenylyl sulfate + desulfo-5-methylthiopentylglucosinolate
adenosine 3',5'-bisphosphate + 5-methylthiopentylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-6-methylthiohexylglucosinolate
adenosine 3',5'-bisphosphate + 6-methylthiohexylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptylglucosinolate
adenosine 3',5'-bisphosphate + 7-methylthioheptylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctylglucosinolate
adenosine 3',5'-bisphosphate + 8-methylthiooctylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-benzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
3'-phosphoadenylyl sulfate + desulfo-indole-3-methylglucosinolate
adenosine 3',5'-bisphosphate + indole-3-methylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutylglucosinolate
adenosine 3',5'-bisphosphate + 4-methylthiobutylglucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
preferred substrate of isozyme SOT18 of ecotype C24
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
preferred substrate of isozyme SOT18 of ecotype C24
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
-
-
?
3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
preferred substrate of isozyme SOT16 of ecotype C24
-
?
adenosine 3',5'-bisphosphate + glucoiberverin
?
-
-
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview
-
?
additional information
?
-
enzyme prefers long-chained methionine-derived desulfoglucosinolates desulfo4-methythiobutylglucosinolate and desulfo-5-methylthiopentylglucosinoate
-
?
additional information
?
-
-
enzyme prefers long-chained methionine-derived desulfoglucosinolates desulfo4-methythiobutylglucosinolate and desulfo-5-methylthiopentylglucosinoate
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
-
?
additional information
?
-
qualitative glucosinolate analysis in leaves of ecotype C24, overview
-
-
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.055
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0
0.07
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT16
0.088
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT17
0.1
desulfo-3-methylthiopropylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype C24
0.043
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0
0.065
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT17
0.08
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT16
0.13
desulfo-4-methylthiobutylglucosinolate
pH 9.0, 37°C, isozyme SOT18, ecotype C24
additional information
additional information
kinetics, overview
-
additional information
additional information
kinetics, overview
-
additional information
additional information
kinetics, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype C24 and ecotype Col0 differ in exchanges D301G and K339N
UniProt
brenda
ecotype C24 and ecotype Col0 differ in exchanges W183R and S344T
UniProt
brenda
isozyme SOT18; ecotypes C24 and Columbia-0, isozyme SOT18
UniProt
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
low expression in plants up to 4 weeks old and slightly elevated in the oldest plants harvested in weeks 5 and 6
brenda
mature leaf of the primary rosette, high expression
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SOT17_ARATH
346
0
39912
Swiss-Prot
other Location (Reliability: 2)
SOT18_ARATH
350
0
40465
Swiss-Prot
other Location (Reliability: 2)
SOT16_ARATH
338
0
39219
Swiss-Prot
other Location (Reliability: 2)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
G301D
exchange of amino acid from ecotype C24 to that of ecotype Col0. 25fold increase in specific activity with desulfobenzylglucosinolate
N339K
exchange of amino acid from ecotype C24 to that of ecotype Col0, residue glycine at position 301 may play a critical role in substrate recognition
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant isozyme SOT16 from Escherichia coli by affinity chromatography
recombinant isozyme SOT17 from Escherichia coli by affinity chromatography
recombinant isozymes SOT18 from Escherichia coli by affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
overexpression of isozyme SOT16 from ecotype C24 in Escherichia coli
overexpression of isozymes SOT17 from ecotype C24 in Escherichia coli
overexpression of the isozymes SOT18 from ecotype C24 and ecotype Columbia-0 in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the highest mRNA levels are determined at the end of the light phase, and the lowest at the end of dark phase
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Klein, M.; Papenbrock, J.
Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana
Physiol. Plant.
135
140-149
2009
Arabidopsis thaliana (Q9C9C9), Arabidopsis thaliana (Q9C9D0), Arabidopsis thaliana (Q9FZ80)
brenda
Luczak, S.; Forlani, F.; Papenbrock, J.
Desulfo-glucosinolate sulfotransferases isolated from several Arabidopsis thaliana ecotypes differ in their sequence and enzyme kinetics
Plant Physiol. Biochem.
63
15-23
2013
Arabidopsis thaliana, Arabidopsis thaliana ecotype C24, Arabidopsis thaliana ecotype Cvi-0, Arabidopsis thaliana ecotype Col-0, Arabidopsis thaliana ecotype Ws, Arabidopsis thaliana ecotype Bla-10, Arabidopsis thaliana ecotype Ler-1
brenda
Klein, M.; Reichelt, M.; Gershenzon, J.; Papenbrock, J.
The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed
FEBS J.
273
122-136
2006
Arabidopsis thaliana (Q9C9C9), Arabidopsis thaliana (Q9FZ80)
-
brenda
Piotrowski, M.; Schemenewitz, A.; Lopukhina, A.; Müller, A.; Janowitz, T.; Weiler, E.; Oecking, C.
Desulfoglucosinolate sulfotransferases from Arabidopsis thaliana catalyze the final step in the biosynthesis of the glucosinolate core structure
J. Biol. Chem.
279
50717-50725
2004
Arabidopsis thaliana (Q9C9C9), Arabidopsis thaliana (Q9FZ80)
brenda
Hirschmann, F.; Papenbrock, J.
The fusion of genomes leads to more options A comparative investigation on the desulfo-glucosinolate sulfotransferases of Brassica napus and homologous proteins of Arabidopsis thaliana
Plant Physiol. Biochem.
91
10-19
2015
Brassica napus (A0A0K1H0V2), Brassica napus
brenda
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