Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
G301D | exchange of amino acid from ecotype C24 to that of ecotype Col0. 25fold increase in specific activity with desulfobenzylglucosinolate | Arabidopsis thaliana |
N339K | exchange of amino acid from ecotype C24 to that of ecotype Col0, residue glycine at position 301 may play a critical role in substrate recognition | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.06 | - |
3'-phosphoadenylyl sulfate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.1 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.25 | - |
3'-phosphoadenylyl sulfate | pH 9.0, 30°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C9C9 | ecotype C24 and ecotype Col0 differ in exchanges D301G and K339N | - |
Arabidopsis thaliana | Q9FZ80 | ecotype C24 and ecotype Col0 differ in exchanges W183R and S344T | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | low expression in plants up to 4 weeks old and slightly elevated in the oldest plants harvested in weeks 5 and 6 | Arabidopsis thaliana | - |
leaf | mature leaf of the primary rosette, high expression | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
root | highest transcription rate | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + desulfo-2-phenylethyl glucosinolate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 2-phenylethyl glucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfo-6-methylthiohexylglucosinolate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 6-methylthiohexylglucosinolate | - |
? | |
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + benzylglucosinolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39900, calculated | Arabidopsis thaliana |
? | x * 40500, calculated | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
cytosolic sulfotransferase 17 | - |
Arabidopsis thaliana |
cytosolic sulfotransferase 18 | - |
Arabidopsis thaliana |
SOT17 | - |
Arabidopsis thaliana |
SOT18 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.0058 | - |
3'-phosphoadenylyl sulfate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.0352 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.0355 | - |
3'-phosphoadenylyl sulfate | pH 9.0, 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | the highest mRNA levels are determined at the end of the light phase, and the lowest at the end of dark phase | additional information |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.23 | - |
3'-phosphoadenylyl sulfate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.59 | - |
3'-phosphoadenylyl sulfate | pH 9.0, 30°C | Arabidopsis thaliana | |
0.7 | - |
desulfobenzylglucosinolate | pH 9.0, 30°C | Arabidopsis thaliana |