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Literature summary for 2.8.2.38 extracted from

  • Klein, M.; Reichelt, M.; Gershenzon, J.; Papenbrock, J.
    The three desulfoglucosinolate sulfotransferase proteins in Arabidopsis have different substrate specificities and are differentially expressed (2006), FEBS J., 273, 122-136 .
No PubMed abstract available
Search for more literature for 2.8.2.38

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
G301D exchange of amino acid from ecotype C24 to that of ecotype Col0. 25fold increase in specific activity with desulfobenzylglucosinolate Arabidopsis thaliana
N339K exchange of amino acid from ecotype C24 to that of ecotype Col0, residue glycine at position 301 may play a critical role in substrate recognition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 desulfobenzylglucosinolate pH 9.0, 30°C Arabidopsis thaliana
0.06
-
 3'-phosphoadenylyl sulfate pH 9.0, 30°C Arabidopsis thaliana
0.1
-
 desulfobenzylglucosinolate pH 9.0, 30°C Arabidopsis thaliana
0.25
-
 3'-phosphoadenylyl sulfate pH 9.0, 30°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Arabidopsis thaliana 5737
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9C9C9 ecotype C24 and ecotype Col0 differ in exchanges D301G and K339N
-
Arabidopsis thaliana Q9FZ80 ecotype C24 and ecotype Col0 differ in exchanges W183R and S344T
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf low expression in plants up to 4 weeks old and slightly elevated in the oldest plants harvested in weeks 5 and 6 Arabidopsis thaliana
-
leaf mature leaf of the primary rosette, high expression Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
root highest transcription rate Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + desulfo-2-phenylethyl glucosinolate
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 2-phenylethyl glucosinolate
-
 ?
3'-phosphoadenylyl sulfate + desulfo-6-methylthiohexylglucosinolate
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + 6-methylthiohexylglucosinolate
-
 ?
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate
-
 Arabidopsis thaliana adenosine 3',5'-bisphosphate + benzylglucosinolate
-
 ?

Subunits

Subunits Comment Organism
? x * 39900, calculated Arabidopsis thaliana
? x * 40500, calculated Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
cytosolic sulfotransferase 17
-
 Arabidopsis thaliana
cytosolic sulfotransferase 18
-
 Arabidopsis thaliana
SOT17
-
 Arabidopsis thaliana
SOT18
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0051
-
 desulfobenzylglucosinolate pH 9.0, 30°C Arabidopsis thaliana
0.0058
-
 3'-phosphoadenylyl sulfate pH 9.0, 30°C Arabidopsis thaliana
0.0352
-
 desulfobenzylglucosinolate pH 9.0, 30°C Arabidopsis thaliana
0.0355
-
 3'-phosphoadenylyl sulfate pH 9.0, 30°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the highest mRNA levels are determined at the end of the light phase, and the lowest at the end of dark phase additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
 desulfobenzylglucosinolate pH 9.0, 30°C Arabidopsis thaliana
0.23
-
 3'-phosphoadenylyl sulfate pH 9.0, 30°C Arabidopsis thaliana
0.59
-
 3'-phosphoadenylyl sulfate pH 9.0, 30°C Arabidopsis thaliana
0.7
-
 desulfobenzylglucosinolate pH 9.0, 30°C Arabidopsis thaliana