EC Number |
Protein Variants |
Reference |
---|
2.7.9.1 | D280A |
partial activity |
645463 |
2.7.9.1 | E279A |
partial activity |
645463 |
2.7.9.1 | G525A |
site-directed mutagenesis, the mutant shows no phosphorylation signal |
739352 |
2.7.9.1 | G525P |
site-directed mutagenesis, the mutant shows no phosphorylation signal |
739352 |
2.7.9.1 | H458N |
no activity |
645460, 645466 |
2.7.9.1 | H529A |
site-directed mutagenesis |
739352 |
2.7.9.1 | more |
35 kDa C-terminal deletion mutant catalyzes the formation of a diphosphorylenzyme intermediate and diphosphate, but not the subsequent formation of phosphoenolpyruvate. A 25 kDa N-terminal deletion mutant catalyzes the second partial reaction but not the first one |
645450 |
2.7.9.1 | more |
application of ribozyme-mediated cleavage of the PPDK transcript to decrease PPDK transcript levels to 20% of normal level with an accompanying decrease in PPDK enzyme activity and decreased ATP levels to 3% of normal levels. Extracellular cleavage of PPDK mRNA by PPDK antisense RNA containing hammerhead ribozyme, overview |
690711 |
2.7.9.1 | more |
construction of a PPDK deletion mutant, DELTAppdk, and a DELTAppdk/DELTApepck double deletion mutant |
-, 738616 |
2.7.9.1 | more |
construction of an enzyme deletion mutant DELTAppdK from strain 2308, and of a double deletion mutant DELTApckADELTAppdK, also lacking phosphoenolpyruvate carboxykinase, both show a reduced growth phenotype, overview. But albeit impaired in growth, the DELTAppdK is still able to reach the replicative intracellular niche, but BABDELTAppdK fails to reach the chronic steady phase typical of virulent brucellae |
-, 738533 |