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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84K203E site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 720462
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84K212A the mutant has strongly impaired catalytic activity, KM for ATP is increased by almost 4fold relative to that of the wild-type protein, and kcat is decreased by roughly 8fold 720462
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84K41E site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 720462
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84K59E site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 720462
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84more construction of enzyme mutants, ChOAS-L-A-FL, which is ChOAS-L-A conjugated with the Flag-Tag DYKDDDDK sequence, is used for generation of eight mutations: The DELTALxxxP domain, DELTAP-LooP domain, DELTAD-D box domain, 5 KR-RR-K193H (one AA substitution), DELTAKR-RR, and ChOAS-L-B (the other allele) are generated by site-directed mutagenesis. The other two mutations, DELTAUbL2 domain and DELTAUbL1/UbL2 domains, are generated in addition. All mutated variants are enzymatically inactive except for ChOAS-L-ADELTAUbL2, but all mutants shows antiviral activity to inhibit the replication of the WNV replicon except for the ChOAS-L-ADELTAUbL1/UbL2, which shows a partial inhibition compared to the wild-type ChOAS-L-A or the other mutant enzyme variants 761657
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84more enzyme OAS1 knockdown by siRNA, or overexpression in Marc-145 cells. Three siRNAs are designed according to the published Macaque OAS1 mRNA sequence (Macaca mulatta, UniProt ID A4LAA0). Knockdown of OAS1 by siRNA significantly increases the frequency of CPE of PRRSV in Marc-145 cells 760363
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84more OAS2 knockdown increases ZIKV replication. Overexpression of OAS2 modestly increases the IFN-induced p-STAT1 both with IFN treatment and without IFN treatment compared to control. OAS2 overexpression also increases ISRE activity and some classical ISGs expression including Myxovirus resistance-A (MxA) and interferon-induced protein 2 (IFIT2) 762508
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84more OASL knockdown via siRNA 761910
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84more substitutions of amino acids in front of the P-loop of Lys42 and Lys60 as well as that of Arg195 reduce the 2'-5'OAS activity at least 50fold. Furthermore substitutions in the Lys199 and Lys204 reduce the activity more than a 1000fold 721753
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.84R194E site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme 720462
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