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Literature summary for 2.7.7.84 extracted from

  • Zhao, J.; Feng, N.; Li, Z.; Wang, P.; Qi, Z.; Liang, W.; Zhou, X.; Xu, X.; Liu, B.
    2',5'-Oligoadenylate synthetase 1 (OAS1) inhibits PRRSV replication in Marc-145 cells (2016), Antiviral Res., 132, 268-273 .
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
additional information enzyme OAS1 knockdown by siRNA, or overexpression in Marc-145 cells. Three siRNAs are designed according to the published Macaque OAS1 mRNA sequence (Macaca mulatta, UniProt ID A4LAA0). Knockdown of OAS1 by siRNA significantly increases the frequency of CPE of PRRSV in Marc-145 cells Chlorocebus sabaeus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Chlorocebus sabaeus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 ATP Chlorocebus sabaeus
-
 pppA2'p5'A2'p5'A + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorocebus sabaeus A0A0D9S3Y6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Marc-145 cell
-
 Chlorocebus sabaeus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 ATP
-
 Chlorocebus sabaeus pppA2'p5'A2'p5'A + 2 diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
2',5'-oligoadenylate synthetase 1
-
 Chlorocebus sabaeus
OAS1
-
 Chlorocebus sabaeus

Expression

Organism Comment Expression
Chlorocebus sabaeus porcine reproductive and respiratory syndrome virus (PRRSV) infection leads to induction of 2',5'-oligoadenylate synthetase gene 1 (OAS1), an important interferon-stimulated gene up

General Information

General Information Comment Organism
malfunction knockdown of endogenous OAS1 increases the PRRSV ORF7 mRNA level to 1.6 and 1.7times of that in control in 24 and 36 hours post-infection of PRRSV, respectively, and leads to approximate 5.5times increase of the frequency of fluorescence positive cells compared to negative control. Overexpression of OAS1 in Marc-145 cells can inhibit the replication of PRRSV Chlorocebus sabaeus
physiological function 2',5'-oligoadenylate synthetase 1 (OAS1) inhibits PRRSV replication in monkey Marc-145 cells. Antiviral activity of OAS1 has been demonstrated in mice and humans with infections of numerous RNA and DNA viruses, such as coxsackie virus, West Nile virus, Hepatitis C virus, cowpox virus, and SARS virus Chlorocebus sabaeus