EC Number   |
Protein Variants |
Reference |
|---|
    2.7.1.164 | D146A |
mutant is active in vivo |
-, 723288 |
| 2.7.1.164 | D41A |
strongly reduced activity |
694429 |
| 2.7.1.164 | G14W |
strongly reduced activity |
-, 694429 |
| 2.7.1.164 | K142A |
mutant is active in vivo |
-, 723288 |
| 2.7.1.164 | K142A/Y143A |
mutant shows severely reduced activity with the Methanopyrus kandleri tRNASec substrate |
723288 |
| 2.7.1.164 | K17A |
mutation abolishes catalytic activity and and inhibits tRNASec recognition |
723288 |
| 2.7.1.164 | K17A |
strongly reduced activity |
-, 694429 |
| 2.7.1.164 | K30A |
mutant is defective in phosphorylation activity |
-, 723288 |
| 2.7.1.164 | more |
truncation of the C-termional domain. Deletions of up to 98 amino acids, PSTK1-153, still show activity, albeit at a much reduced level of about 6% of the initial velocity of the intact enzyme. In vivo, PSTK1-153 is still able compensate for the Escherichia coli selA deletion. Truncation PSTK1-192 lacks the entire CTD domain and exhibits 40% residual activity. PSTK1-215 and PSTK1-240 mutants lack the terminal two and one helices of the helix bundle, respectively. PSTK1-215 mutant shows remarkably reduced activity |
-, 723288 |
| 2.7.1.164 | N161A |
mutant is defective in phosphorylation activity |
723288 |
