Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
D41A | strongly reduced activity | Methanocaldococcus jannaschii |
G14W | strongly reduced activity | Methanocaldococcus jannaschii |
K17A | strongly reduced activity | Methanocaldococcus jannaschii |
R116A | mutant enzyme is 23.5fold less active than wild-type enzyme | Methanocaldococcus jannaschii |
R120A | strongly reduced activity | Methanocaldococcus jannaschii |
S18A | strongly reduced activity | Methanocaldococcus jannaschii |
T19W | mutant enzyme is 2.8fold less active than wild-type enzyme | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00004 | - |
L-seryl-tRNASec | 37°C | Methanocaldococcus jannaschii | |
2.6 | - |
ATP | 37°C | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-seryl-tRNASec | Methanocaldococcus jannaschii | - |
ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | Methanocaldococcus jannaschii DSM 2661 | - |
ADP + O-phospho-L-seryl-tRNASec | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58933 | MJ1538 | - |
Methanocaldococcus jannaschii DSM 2661 | Q58933 | MJ1538 | - |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-seryl-tRNASec | - |
Methanocaldococcus jannaschii | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | the enzyme does not recognize free Ser as a substrate for phosphate transfer. Ser attached to tRNASec is its obligate substrate. Neither tRNASer nor L-seryl-tRNASer is a substrate for phosphorylation | Methanocaldococcus jannaschii | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | - |
Methanocaldococcus jannaschii DSM 2661 | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
ATP + L-seryl-tRNASec | the enzyme does not recognize free Ser as a substrate for phosphate transfer. Ser attached to tRNASec is its obligate substrate. Neither tRNASer nor L-seryl-tRNASer is a substrate for phosphorylation | Methanocaldococcus jannaschii DSM 2661 | ADP + O-phospho-L-seryl-tRNASec | - |
? | |
CTP + L-seryl-tRNASec | phosphorylation at about 60% the activity of ATP | Methanocaldococcus jannaschii | CDP + O-phospho-L-seryl-tRNASec | - |
? | |
CTP + L-seryl-tRNASec | phosphorylation at about 60% the activity of ATP | Methanocaldococcus jannaschii DSM 2661 | CDP + O-phospho-L-seryl-tRNASec | - |
? | |
dATP + L-seryl-tRNASec | phosphorylation at about 65% the activity of ATP | Methanocaldococcus jannaschii | dADP + O-phospho-L-seryl-tRNASec | - |
? | |
dATP + L-seryl-tRNASec | phosphorylation at about 65% the activity of ATP | Methanocaldococcus jannaschii DSM 2661 | dADP + O-phospho-L-seryl-tRNASec | - |
? | |
GTP + L-seryl-tRNASec | phosphorylation at about 40% the activity of ATP | Methanocaldococcus jannaschii | GDP + O-phospho-L-seryl-tRNASec | - |
? | |
ITP + L-seryl-tRNASec | phosphorylation at about 85% the activity of ATP | Methanocaldococcus jannaschii | IDP + O-phospho-L-seryl-tRNASec | - |
? | |
additional information | no phosphorylation activity with 5'-adenylyl (beta,gamma-methylene)diphosphonate, very low phosphorylation activity with alpha,beta-methyleneadenosine 5'-triphosphate | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | no phosphorylation activity with 5'-adenylyl (beta,gamma-methylene)diphosphonate, very low phosphorylation activity with alpha,beta-methyleneadenosine 5'-triphosphate | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? | |
UTP + L-seryl-tRNASec | phosphorylation at about 40% the activity of ATP | Methanocaldococcus jannaschii | UDP + O-phospho-L-seryl-tRNASec | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
ATP | 37°C | Methanocaldococcus jannaschii | |
0.098 | - |
L-seryl-tRNASec | 37°C | Methanocaldococcus jannaschii |