EC Number |
Protein Variants |
Reference |
---|
2.3.3.14 | more |
activity and protein level of homocitrate synthase are dramatically reduced upon depletion of CuZn-superoxide dismutase. Overexpression of the lys4 gene increases homocitrate synthase activity and is sufficient to suppress the lysine requirement of CuZn-superoxide dismutase-deficient cells |
674553 |
2.3.3.14 | H309N |
inactive mutant enzyme |
685263 |
2.3.3.14 | H309A |
inactive mutant enzyme. Slight increase in activity is observed for H309A in the presence of 300 mM imidazole, which is still 1000fold lower than that of wild type |
685263 |
2.3.3.14 | D123N |
mutant |
704648 |
2.3.3.14 | E222Q |
mutant |
704648 |
2.3.3.14 | Q364R |
mutant |
704648 |
2.3.3.14 | R288K |
mutant |
704648 |
2.3.3.14 | E155A |
mutant enzyme exhibits 1000fold lower activity than wild-type enzyme. Activity of E155A can be partially rescued by formate |
685263 |
2.3.3.14 | E155Q |
mutant enzyme exhibits 1000fold lower activity than wild-type enzyme. The kcat for E155Q decreases at high pH, similar to the wild-type enzyme, but is pH independent at low pH |
685263 |
2.3.3.14 | Y320F |
mutant enzyme loses 25fold activity compared to that of the wild-type enzyme |
685263 |