Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.14 extracted from

  • Bulfer, S.L.; Scott, E.M.; Pillus, L.; Trievel, R.C.
    Structural basis for L-lysine feedback inhibition of homocitrate synthase (2010), J. Biol. Chem., 285, 10446-10453.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Schizosaccharomyces pombe

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure of SpHCS in complex with the inhibitor L-lysine and Zn2+ is solved at a resolution of 2.38 A Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
D123N mutant Schizosaccharomyces pombe
E222Q mutant Schizosaccharomyces pombe
Q364R mutant Schizosaccharomyces pombe
R288K mutant Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
L-lysine feedback inhibition Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0107
-
 acetyl-CoA wild-type Schizosaccharomyces pombe
0.0132
-
 acetyl-CoA mutant D123N Schizosaccharomyces pombe
0.014
-
 acetyl-CoA mutant R288K Schizosaccharomyces pombe
0.0153
-
 acetyl-CoA mutant Q364R Schizosaccharomyces pombe
0.0477
-
 acetyl-CoA mutant E222Q Schizosaccharomyces pombe
0.159
-
 2-oxoglutarate wild-type Schizosaccharomyces pombe
0.247
-
 2-oxoglutarate mutant R288K Schizosaccharomyces pombe
0.284
-
 2-oxoglutarate mutant D123N Schizosaccharomyces pombe
0.296
-
 2-oxoglutarate mutant Q364R Schizosaccharomyces pombe
9.66
-
 2-oxoglutarate mutant E222Q Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate Schizosaccharomyces pombe
-
 (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe Q9Y823
-
-

Purification (Commentary)

Purification (Comment) Organism
on Talon or Zn2+ charged IMAC sepharose resin Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + 2-oxoglutarate
-
 Schizosaccharomyces pombe (R)-2-hydroxybutane-1,2,4-tricarboxylate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
HCS
-
 Schizosaccharomyces pombe
homocitrate synthase
-
 Schizosaccharomyces pombe
SpHCS
-
 Schizosaccharomyces pombe

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.75
-
 2-oxoglutarate mutant Q364R Schizosaccharomyces pombe
2.833
-
 acetyl-CoA mutant Q364R Schizosaccharomyces pombe
3.183
-
 2-oxoglutarate mutant E222Q Schizosaccharomyces pombe
3.233
-
 acetyl-CoA mutant R288K Schizosaccharomyces pombe
3.333
-
 2-oxoglutarate mutant R288K Schizosaccharomyces pombe
3.583
-
 acetyl-CoA mutant E222Q Schizosaccharomyces pombe
3.617
-
 acetyl-CoA mutant D123N Schizosaccharomyces pombe
3.617
-
 2-oxoglutarate mutant D123N Schizosaccharomyces pombe
4.983
-
 acetyl-CoA wild-type Schizosaccharomyces pombe
5.133
-
 2-oxoglutarate wild-type Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
 Schizosaccharomyces pombe