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EC Number Protein Variants Commentary Reference
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179C164A site-directed mutagenesis, inactive mutant 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179C164A/H337A site-directed mutagenesis, inactive mutant 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179C164A/K332A site-directed mutagenesis, inactive mutant 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179E346A site-directed mutagenesis, the mutant shows similar activity compared to the wild-type enzyme 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179E396A site-directed mutagenesis, the mutant shows no condensation activity but retains about 50% of wild-type transacylation activity with acyl-ACP and ACP, and 40% of wild-type decarboxylation activity 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179H303A site-directed mutagenesis, the mutant shows 74% reduced condensation activity, 40% reduced transacylation activity, and 5fold increased decarboxylation activity, compared to the wild-type enzyme 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179H337A site-directed mutagenesis, inactive mutant 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179K332A site-directed mutagenesis, the mutant shows no condensation activity but retains about 30% of wild-type transacylation activity with acyl-ACP and ACP, and 10% of wild-type decarboxylation activity 674581
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179C163Q site-directed mutagenesis, interaction with platensimycin compared to the interaction with the wild-type enzyme 676148
Show all pathways known for 2.3.1.179Display the word mapDisplay the reaction diagram Show all sequences 2.3.1.179more construction of KASII knockout mutants by T-DNA disruption, knockout alleles fab1-1 and fab1-2, strong seed-specific hairpin-RNAi reductions in FAB1 expression resulted in abortion of about 1/4 of the embryos in an apparent phenocopy of fab1-2 homozygosity, in less severe FAB1 hairpin-RNAi individuals, embryos developed normally and exhibited a 1:2:1 segregation ratio for palmitate accumulation, thus, early embryo development appears sensitive to elevated 16:0, whereas at later stages, up to 53% of 16:0, i.e. a 7-fold increase over wild-type levels, is tolerated, Fab1-1 mutant plants show about 60% of wild-type enzyme activity and about 17% palm-like oil accumulation compared to the wild-type plants, phenotypes, overview 676894
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