EC Number   |
Protein Variants |
Reference |
|---|
    1.7.3.3 | A132C/A225C |
the mutant shows reduced catalytic efficiency compared to the wild type enzyme |
764897 |
| 1.7.3.3 | A296V |
about 50% ioncrease in catalytic efficiency |
724483 |
| 1.7.3.3 | A89T/G91A7V92M/H245L/E252A/M253I/R291K/A296V/A301S/K303R |
replacement with the corresponding residues of human enzyme. About 30% of wild-type catalytic efficiency |
724483 |
| 1.7.3.3 | C249S |
the substitution has no effect on enzymatic activity |
765701 |
| 1.7.3.3 | C249S/DELTAL302 |
the combination of both mutations increases the enzymatic activity by 8%.Tthe specific activity of the mutant enzyme does not decrease after exposed to 37°C for 20 h |
765701 |
| 1.7.3.3 | D288C |
the mutant shows 0.8fold reduced catalytic efficiency compared to the wild type enzyme |
764897 |
| 1.7.3.3 | D307R |
mutant is an inactive homotetramer of lower solubility |
-, 741750 |
| 1.7.3.3 | D79A |
the KM-value for urate is 82% of the wild-type value |
657986 |
| 1.7.3.3 | DELTAL302 |
the mutant has improved specific activity (6%) compared with wild type enzyme |
765701 |
| 1.7.3.3 | E318R |
mutant is similar to S314Stop |
741750 |
