Literature summary for 1.7.3.3 extracted from

Tao, L.; Li, D.; Li, Y.; Shi, X.; Wang, J.; Rao, C.; Zhang, Y.
Designing a mutant Candida uricase with improved polymerization state and enzymatic activity (2017), Protein Eng. Des. Sel., 30, 753-759 .

Search for more literature for 1.7.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Candida sp. (in: Saccharomycetales)

Protein Variants

Protein Variants	Comment	Organism
C249S	the substitution has no effect on enzymatic activity	Candida sp. (in: Saccharomycetales)
C249S/DELTAL302	the combination of both mutations increases the enzymatic activity by 8%.Tthe specific activity of the mutant enzyme does not decrease after exposed to 37°C for 20 h	Candida sp. (in: Saccharomycetales)
DELTAL302	the mutant has improved specific activity (6%) compared with wild type enzyme	Candida sp. (in: Saccharomycetales)

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
peroxisome	-	Candida sp. (in: Saccharomycetales)	5777	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
urate + O2 + H2O	Candida sp. (in: Saccharomycetales)	-	5-hydroxyisourate + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Candida sp. (in: Saccharomycetales)	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Q column chromatography and Superdex 200 gel filtration	Candida sp. (in: Saccharomycetales)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
urate + O2 + H2O	-	Candida sp. (in: Saccharomycetales)	5-hydroxyisourate + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
uricase	-	Candida sp. (in: Saccharomycetales)

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	the specific activity of wild type enzyme decreases to 90% after exposed to 37°C for 20h	Candida sp. (in: Saccharomycetales)

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Release 2023.1 (January 2023)