EC Number   |
Protein Variants |
Reference |
|---|
   1.2.3.1 | A1023Y |
significant decrease in activity on methotrexate |
740025 |
| 1.2.3.1 | A1023Y |
the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme |
711838 |
| 1.2.3.1 | A1081V |
the mutant completely loses the high cinchonidine oxidation activity of the wild type enzyme |
711838 |
| 1.2.3.1 | A1083T |
the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme |
711838 |
| 1.2.3.1 | A1083T/V1085A |
the mutant shows increased Km towards (+)-4-(4-cyanoanilino)-5,6-dihydro-7-hydroxy-7H-cyclopenta-[d]-pyrimidine compared to the wild type enzyme |
711838 |
| 1.2.3.1 | A807V |
does not affect the kinetic constants with smaller substrates like benzaldehyde or phthalazine, but affinity for bulkier substrates like phenanthridine decreases, whereas the catalytic efficiency is slightly raised |
725498 |
| 1.2.3.1 | E1265Q |
catalytically inactive, residue E1265 initiates the base-catalyzed mechanism of substrate oxidation |
700901 |
| 1.2.3.1 | E1266Q |
complete loss of activity with different N-heterocyclic compounds as substrates, 60% reduction of enzyme activity with benzaldehyde |
725498 |
| 1.2.3.1 | F1014I |
amino acid exchange in the active site |
741285 |
| 1.2.3.1 | F1014L |
amino acid exchange in the active site, 10fold increase in molybdenim content |
741285 |
