Crystallization (Comment) | Organism |
---|---|
to 2.9 A resolution, used for elucidation of putative reaction mechanism of aldehyde oxidases | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
A807V | does not affect the kinetic constants with smaller substrates like benzaldehyde or phthalazine, but affinity for bulkier substrates like phenanthridine decreases, whereas the catalytic efficiency is slightly raised | Mus musculus |
E1266Q | complete loss of activity with different N-heterocyclic compounds as substrates, 60% reduction of enzyme activity with benzaldehyde | Mus musculus |
K889H | 23fold decrease in the catalytic efficiency using benzaldehyde and phthalazine as substrates, but the Km-values remain the same | Mus musculus |
Y885M | kinetic constants remain mainly the same with small hydrophobic substrates like benzaldehyde and phthalazine, bulkier substrates like phenanthridine or more charged substrates like N1-methylnicotinamide are converted with higher efficiency | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | - |
Mus musculus | |
menadione | - |
Mus musculus | |
norharmane | - |
Mus musculus | |
raloxifene | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | further kinetic parameters of phthalazine-DCPIP reaction in the absence or presence of the inhibitors benzamidine, menadione, norharmane, and raloxifene available in the publication | Mus musculus | |
0.0011 | - |
Phthalazine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.0014 | - |
Phthalazine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.0023 | - |
benzaldehyde | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.0025 | - |
benzaldehyde | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.0027 | - |
Phthalazine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
0.0029 | - |
benzaldehyde | A807V mutant, pH 8.0, 37°C | Mus musculus | |
0.0029 | - |
Phthalazine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.0032 | - |
Phthalazine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
0.0046 | - |
benzaldehyde | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.0063 | - |
benzaldehyde | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
0.0118 | - |
phenanthridine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.0161 | - |
phenanthridine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.0255 | - |
N1-methylnicotinamide | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.0323 | - |
phenanthridine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.0334 | - |
phenanthridine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
0.0359 | - |
N1-methylnicotinamide | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.0827 | - |
N1-methylnicotinamide | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
0.0863 | - |
benzaldehyde | E1266Q mutant, pH 8.0, 37°C | Mus musculus | |
0.1234 | - |
N1-methylnicotinamide | A807V mutant, pH 8.0, 37°C | Mus musculus | |
0.1285 | - |
N1-methylnicotinamide | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.1495 | - |
phenanthridine | A807V mutant, pH 8.0, 37°C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | G3X982 | - |
- |
Purification (Comment) | Organism |
---|---|
mAOX3 purified from CD1 mouse liver as well as from a heterologous expression system from Escherichia coli, recombinant mAOX3 expressed as an N-terminal fusion protein with a His6 tag | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzaldehyde + 2,6-dichlorophenol indophenol | natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol | Mus musculus | ? | - |
? | |
N1-methylnicotinamide + 2,6-dichlorophenol indophenol | natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol | Mus musculus | ? | - |
? | |
phenanthridine + H2O + O2 | - |
Mus musculus | ? | - |
? | |
phthalazine + 2,6-dichlorophenol indophenol | natural electron acceptor of enzyme is molecular oxygen, DCPIP i.e., 2,6-dichlorophenol indophenol | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | each monomer comprises 1335 residues and can be divided into three major domains, a small 20000 Da N-terminal domain that harbors the two FeS clusters and connects to the 40000 Da FAD binding domain and finally the C-terminal 90000 Da domain containing the Moco binding site, with the molybdenum catalytic center | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
AOX3 | - |
Mus musculus |
mAOX3 | - |
Mus musculus |
mouse liver aldehyde oxidase 3 | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | further kinetic parameters of phthalazine-DCPIP reaction in the absence or presence of the inhibitors benzamidine, menadione, norharmane, and raloxifene available in the publication | Mus musculus | |
0.052 | - |
N1-methylnicotinamide | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.17 | - |
benzaldehyde | E1266Q mutant, pH 8.0, 37°C | Mus musculus | |
0.187 | - |
N1-methylnicotinamide | A807V mutant, pH 8.0, 37°C | Mus musculus | |
0.197 | - |
Phthalazine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.245 | - |
N1-methylnicotinamide | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.32 | - |
benzaldehyde | K889H mutant, pH 8.0, 37°C | Mus musculus | |
0.39 | - |
N1-methylnicotinamide | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
0.45 | - |
N1-methylnicotinamide | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.683 | - |
benzaldehyde | A807V mutant, pH 8.0, 37°C | Mus musculus | |
0.685 | - |
Phthalazine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
0.685 | - |
Phthalazine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.69 | - |
Phthalazine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.698 | - |
benzaldehyde | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.727 | - |
benzaldehyde | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
0.815 | - |
Phthalazine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
0.862 | - |
phenanthridine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
0.957 | - |
benzaldehyde | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
1.33 | - |
phenanthridine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
3.63 | - |
phenanthridine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
4.45 | - |
phenanthridine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
5.25 | - |
phenanthridine | A807V mutant, pH 8.0, 37°C | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mus musculus | |
iron-sulfur centre | two centers, FeSI and FeSII | Mus musculus | |
molybdenum cofactor | - |
Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
menadione | uncompetitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
0.0122 | - |
raloxifene | competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
0.018 | - |
norharmane | competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
0.0225 | - |
benzamidine | competitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus | |
0.0591 | - |
raloxifene | uncompetitive inhibition of phthalazine-2,6-dichlorophenol indophenol reaction, pH 8.0, 37°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.52 | - |
N1-methylnicotinamide | A807V mutant, pH 8.0, 37°C | Mus musculus | |
1.92 | - |
N1-methylnicotinamide | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
1.97 | - |
benzaldehyde | E1266Q mutant, pH 8.0, 37°C | Mus musculus | |
2 | - |
N1-methylnicotinamide | K889H mutant, pH 8.0, 37°C | Mus musculus | |
4.72 | - |
N1-methylnicotinamide | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
12.5 | - |
N1-methylnicotinamide | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
26.7 | - |
phenanthridine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
35.2 | - |
phenanthridine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
82.8 | - |
phenanthridine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
133.2 | - |
phenanthridine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
136.7 | - |
benzaldehyde | K889H mutant, pH 8.0, 37°C | Mus musculus | |
151.8 | - |
benzaldehyde | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
158 | - |
benzaldehyde | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
175.5 | - |
Phthalazine | K889H mutant, pH 8.0, 37°C | Mus musculus | |
235.7 | - |
benzaldehyde | A807V mutant, pH 8.0, 37°C | Mus musculus | |
238 | - |
Phthalazine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
253.7 | - |
Phthalazine | A807V mutant, pH 8.0, 37°C | Mus musculus | |
254.7 | - |
Phthalazine | Y885M mutant, pH 8.0, 37°C | Mus musculus | |
279.3 | - |
benzaldehyde | mAOX3 wild type, pH 8.0, 37°C | Mus musculus | |
307.8 | - |
phenanthridine | A807V/Y885M double mutant, pH 8.0, 37°C | Mus musculus | |
489.3 | - |
Phthalazine | mAOX3 wild type, pH 8.0, 37°C | Mus musculus |