EC Number   |
Protein Variants |
Reference |
|---|
   1.14.11.2 | C150S |
the mutation has no major effect on tetramer assembly, but the amount of tetramer is slightly reduced, being about 80% of that of the wild-type enzyme |
439238 |
| 1.14.11.2 | C486S |
the mutation totally prevents tetramer assembly |
439238 |
| 1.14.11.2 | C511S |
the mutation totally prevents tetramer assembly |
439238 |
| 1.14.11.2 | D149A |
a mutation at the tip of the betaII-betaIII loop, the mutant shows reduced activity and narrower substrate specificity due to altered substrate binding compared to the wild-type enzyme |
698933 |
| 1.14.11.2 | D149N |
a mutation at the tip of the betaII-betaIII loop, the mutant shows reduced activity and narrower substrate specificity due to altered substrate binding compared to the wild-type enzyme |
698933 |
| 1.14.11.2 | D405 |
inactivation of catalytic site in PHY-2 subunit, 8% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity |
659333 |
| 1.14.11.2 | D407N |
inactivation of catalytic site in PHY-1 subunit, 5% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity |
659333 |
| 1.14.11.2 | D414A |
site-directed mutagenesis, the mutation mimics the active site of a halogenase. The substitutions does not convert P4H into a halogenase, but the hydroxylase activity of D414A P4H cannot be rescued with small molecule, the mutant is inactive |
700909 |
| 1.14.11.2 | D414G |
site-directed mutagenesis, the mutation mimics the active site of a halogenase. The substitutions does not convert P4H into a halogenase, but the hydroxylase activity of D414A P4H cannot be rescued with small molecule, the mutant is inactive |
700909 |
| 1.14.11.2 | D414H |
site-directed mutagenesis, the mutation mimics the active site of a cysteine dioxygenase, the mutant is inactive |
700909 |
