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Literature summary for 1.14.11.2 extracted from

  • Kukkola, L.; Koivunen, P.; Pakkanen, O.; Page, A.P.; Myllyharju, J.
    Collagen prolyl 4-hydroxylase tetramers and dimers show identical decreases in Km values for peptide substrates with increasing chain length: mutation of one of the two catalytic sites in the tetramer inactivates the enzyme by more than half (2004), J. Biol. Chem., 279, 18656-18661.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate required for activity Caenorhabditis elegans

Cloned(Commentary)

Cloned (Comment) Organism
expression of PHY-1/PHY-2/(PDI-2)2 P4H tetramer in insect cells Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
D405 inactivation of catalytic site in PHY-2 subunit, 8% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans
D407N inactivation of catalytic site in PHY-1 subunit, 5% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans
L484A lysine of PHY-2 that binds the C-5 carboxyl group of 2-oxoglutarate, 27% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans
L486A lysine of PHY-1 that binds the C-5 carboxyl group of 2-oxoglutarate, 25% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
510-amino acid alpha1(I) collagen fragment PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans
0.001
-
Fe2+
-
Caenorhabditis elegans
0.006
-
100-amino acid alpha1(I) collagen fragment PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans
0.025
-
(L-Pro-L-Pro-Gly)10 PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans
0.08
-
2-oxoglutarate
-
Caenorhabditis elegans
0.66
-
(L-Pro-L-Pro-Gly)5 PHY-1/PHY-2/(PDI-2)2 P4H tetramer Caenorhabditis elegans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for activity Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen L-proline + 2-oxoglutarate + O2 Caenorhabditis elegans
-
procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2
-
Caenorhabditis elegans (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2
-
r
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2
-
Caenorhabditis elegans (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2
-
r
(Pro-Pro-Gly)10 L-proline + 2-oxoglutarate + O2
-
Caenorhabditis elegans (Pro-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2
-
?
(Pro-Pro-Gly)2 L-proline + 2-oxoglutarate + O2
-
Caenorhabditis elegans (Pro-Pro-Gly)2 trans-4-hydroxy-L-proline + succinate + CO2
-
?
100-amino acid alpha1(I) collagen fragment + 2-oxoglutarate + O2
-
Caenorhabditis elegans ?
-
r
100-amino acid alpha1(I) collagen fragment L-proline + 2-oxoglutarate + O2
-
Caenorhabditis elegans 100-amino acid alpha1(I) collagen fragment trans-4-hydroxy-L-proline + succinate + CO2
-
?
510-amino acid alpha1(I) collagen fragment + 2-oxoglutarate + O2
-
Caenorhabditis elegans ?
-
r
510-amino acid alpha1(I) collagen fragment L-proline + 2-oxoglutarate + O2
-
Caenorhabditis elegans 510-amino acid alpha1(I) collagen fragment trans-4-hydroxy-L-proline + succinate + CO2
-
?
procollagen L-proline + 2-oxoglutarate + O2
-
Caenorhabditis elegans procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
?

Subunits

Subunits Comment Organism
tetramer PHY-1,PHY-2,(PDI)2 subunits Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
P4H
-
Caenorhabditis elegans