Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required for activity | Caenorhabditis elegans |
Cloned (Comment) | Organism |
---|---|
expression of PHY-1/PHY-2/(PDI-2)2 P4H tetramer in insect cells | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
D405 | inactivation of catalytic site in PHY-2 subunit, 8% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity | Caenorhabditis elegans |
D407N | inactivation of catalytic site in PHY-1 subunit, 5% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity | Caenorhabditis elegans |
L484A | lysine of PHY-2 that binds the C-5 carboxyl group of 2-oxoglutarate, 27% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity | Caenorhabditis elegans |
L486A | lysine of PHY-1 that binds the C-5 carboxyl group of 2-oxoglutarate, 25% of wild-type PHY-1/PHY-2/(PDI-2)2 P4H tetramer activity | Caenorhabditis elegans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
510-amino acid alpha1(I) collagen fragment | PHY-1/PHY-2/(PDI-2)2 P4H tetramer | Caenorhabditis elegans | |
0.001 | - |
Fe2+ | - |
Caenorhabditis elegans | |
0.006 | - |
100-amino acid alpha1(I) collagen fragment | PHY-1/PHY-2/(PDI-2)2 P4H tetramer | Caenorhabditis elegans | |
0.025 | - |
(L-Pro-L-Pro-Gly)10 | PHY-1/PHY-2/(PDI-2)2 P4H tetramer | Caenorhabditis elegans | |
0.08 | - |
2-oxoglutarate | - |
Caenorhabditis elegans | |
0.66 | - |
(L-Pro-L-Pro-Gly)5 | PHY-1/PHY-2/(PDI-2)2 P4H tetramer | Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
procollagen L-proline + 2-oxoglutarate + O2 | Caenorhabditis elegans | - |
procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(L-Pro-L-Pro-Gly)10 + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | (L-Pro-L-Pro-Gly)10-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(L-Pro-L-Pro-Gly)5 + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | (L-Pro-L-Pro-Gly)5-trans-4-hydroxy-L-proline + succinate + CO2 | - |
r | |
(Pro-Pro-Gly)10 L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | (Pro-Pro-Gly)10 trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
(Pro-Pro-Gly)2 L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | (Pro-Pro-Gly)2 trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
100-amino acid alpha1(I) collagen fragment + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | ? | - |
r | |
100-amino acid alpha1(I) collagen fragment L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | 100-amino acid alpha1(I) collagen fragment trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
510-amino acid alpha1(I) collagen fragment + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | ? | - |
r | |
510-amino acid alpha1(I) collagen fragment L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | 510-amino acid alpha1(I) collagen fragment trans-4-hydroxy-L-proline + succinate + CO2 | - |
? | |
procollagen L-proline + 2-oxoglutarate + O2 | - |
Caenorhabditis elegans | procollagen trans-4-hydroxy-L-proline + succinate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | PHY-1,PHY-2,(PDI)2 subunits | Caenorhabditis elegans |
Synonyms | Comment | Organism |
---|---|---|
P4H | - |
Caenorhabditis elegans |