EC Number   |
Protein Variants |
Reference |
|---|
   1.11.1.16 | A173R |
kcat/KM for Mn2+ is 1.4fold lower than wild-type value, kcat/Km for veratryl alcohol is 1.4fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.3fold higher than wild-type value |
672267 |
| 1.11.1.16 | A260F |
kinetics similar to wild-type |
685203 |
| 1.11.1.16 | A260F/R257A |
site-directed mutagenesis |
704886 |
| 1.11.1.16 | D153A |
mutation minimally affects the second order rate constant for Compound I formation and the specificity constant for H2O2, but substitution dramatically reduces the stability of Compound I |
725465 |
| 1.11.1.16 | D153A/N246A |
mutation reduces the second order rate constant for Compound I formation and the specificity constant for H2O2 less than 30fold, substitution dramatically reduces the stability of Compound I |
725465 |
| 1.11.1.16 | D153H |
mutant is more than an order of magnitude less reactive with H2O2 than wild-type |
725465 |
| 1.11.1.16 | D175A |
kcat/KM for Mn2+ is 842fold lower than wild-type value, kcat/Km for veratryl alcohol is3.2 fold higher than wild-type value, kcat/Km for Reactive Black 5 is 1.8fold higher than wild-type value |
672267 |
| 1.11.1.16 | D22N/T45A/E83G/I103V/G107S/P141A/F186L |
site-directed mutagenesis |
764058 |
| 1.11.1.16 | E140G |
substitution of bulky residue at the main heme access channel, kinetic analysis |
725499 |
| 1.11.1.16 | E140G/K176G |
variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 |
725499 |
