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Literature summary for 1.11.1.16 extracted from

  • Morales, M.; Mate, M.J.; Romero, A.; Martinez, M.J.; Martinez, A.T.; Ruiz-Duenas, F.J.
    Two oxidation sites for low redox potential substrates: a directed mutagenesis, kinetic, and crystallographic study on Pleurotus eryngii versatile peroxidase (2012), J. Biol. Chem., 287, 41053-41067.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.16

Crystallization (Commentary)

Crystallization (Comment) Organism
mutants E140G, P141G, K176G, and E140G/K176G, to 1.6, 2.0, 1.5, 1.7 and 2.35 A resolution, respectively Pleurotus eryngii

Protein Variants

Protein Variants Comment Organism
E140G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
E140G/K176G variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 Pleurotus eryngii
E140G/P141G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
E140G/P141G/K176G variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 Pleurotus eryngii
E140G/W164S/K176G variant attains catalytic efficiencies for oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at the heme channel similar to those of the exposed tryptophan site W164 Pleurotus eryngii
F142G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
K176D substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
K176G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
K215G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
K215Q substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
P141G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
P76G substitution of bulky residue at the main heme access channel, kinetic analysis Pleurotus eryngii
W164S loss activity. Residue is responsible for high redox potential substrate oxidation Pleurotus eryngii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.003
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.004
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0041
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0054
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0054
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0054
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0059
-
 4-hydroquinone mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.008
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0103
-
 4-hydroquinone mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0122
-
 4-hydroquinone mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.013
-
 4-hydroquinone mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0143
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0156
-
 4-hydroquinone wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0174
-
 4-hydroquinone mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0189
-
 4-hydroquinone mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0194
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0194
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0205
-
 4-hydroquinone mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0251
-
 4-hydroquinone mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.034
-
 catechol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
0.036
-
 2,6-dimethoxyphenol mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0362
-
 4-hydroquinone mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.038
-
 2,6-dimethoxyphenol mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0397
-
 4-hydroquinone mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.04
-
 4-hydroquinone mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.058
-
 2,6-dimethoxyphenol mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.065
-
 2,6-dimethoxyphenol mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.066
-
 2,6-dimethoxyphenol mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.078
-
 2,6-dimethoxyphenol mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.078
-
 2,6-dimethoxyphenol wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.0836
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.1
-
 2,6-dimethoxyphenol mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.104
-
 2,6-dimethoxyphenol mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.119
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.155
-
 2,6-dimethoxyphenol mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.189
-
 2,6-dimethoxyphenol mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.305
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.38
-
 4-hydroquinone mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.383
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.41
-
 4-hydroquinone mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.42
-
 4-hydroquinone mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.461
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.618
-
 4-hydroquinone mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.716
-
 4-hydroquinone wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.828
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.836
-
 4-hydroquinone mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
0.884
-
 4-hydroquinone mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.02
-
 4-hydroquinone mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.026
-
 4-hydroquinone mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.05
-
 4-hydroquinone mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.09
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.112
-
 4-hydroquinone mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.114
-
 4-hydroquinone mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.18
-
 4-hydroquinone mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.66
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.86
-
 catechol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
1.97
-
 guaiacol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
2.23
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.24
-
 4-hydroquinone mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.38
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.56
-
 2,6-dimethoxyphenol mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.63
-
 catechol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
2.73
-
 guaiacol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
2.86
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.97
-
 2,6-dimethoxyphenol mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
3.33
-
 2,6-dimethoxyphenol mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
3.68
-
 catechol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
4.04
-
 catechol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
4.26
-
 catechol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
4.26
-
 catechol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
4.72
-
 catechol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
4.79
-
 catechol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
5.04
-
 catechol wild-type, pH 3.5, 25°C Pleurotus eryngii
5.1
-
 guaiacol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
5.11
-
 catechol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
5.15
-
 catechol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
5.85
-
 guaiacol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
6.9
-
 guaiacol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
7.27
-
 guaiacol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
7.76
-
 catechol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
10.3
-
 guaiacol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
10.5
-
 catechol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
10.5
-
 2,6-dimethoxyphenol wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
10.8
-
 guaiacol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
11.1
-
 guaiacol wild-type, pH 3.5, 25°C Pleurotus eryngii
11.6
-
 guaiacol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
14.2
-
 guaiacol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
16
-
 2,6-dimethoxyphenol mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
16.2
-
 guaiacol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
16.3
-
 guaiacol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
36.2
-
 2,6-dimethoxyphenol mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
37.4
-
 2,6-dimethoxyphenol mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
37.6
-
 2,6-dimethoxyphenol mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
39.8
-
 guaiacol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
76
-
 2,6-dimethoxyphenol mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii

Organism

Organism UniProt Comment Textmining
Pleurotus eryngii O94753 isoform Vpl2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2,6-dimethoxyphenol + 2 H2O2
-
 Pleurotus eryngii coerulignone + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus eryngii ? + H2O
-
 ?
4-hydroquinone + H2O2
-
 Pleurotus eryngii 4-benzoquinone + H2O
-
 ?
catechol + H2O2
-
 Pleurotus eryngii 2-benzoquinone + H2O
-
 ?
guaiacol + H2O2
-
 Pleurotus eryngii 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
additional information presence of two independent catalytic sites for different phenols and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) in native enzyme, characterizedby high and low specificity constants, i.e. Km in the micromolar range and Km in the millimolar range, respcetively Pleurotus eryngii ?
-
 ?

Synonyms

Synonyms Comment Organism
Vpl2
-
 Pleurotus eryngii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 2,6-dimethoxyphenol mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
4.8
-
 2,6-dimethoxyphenol mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
5.6
-
 2,6-dimethoxyphenol wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
6.2
-
 2,6-dimethoxyphenol mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
6.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
7.2
-
 4-hydroquinone mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
7.6
-
 2,6-dimethoxyphenol mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
7.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
8
-
 4-hydroquinone mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
8
-
 4-hydroquinone mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
8
-
 catechol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
8.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
9.3
-
 2,6-dimethoxyphenol mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
9.3
-
 guaiacol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
9.5
-
 2,6-dimethoxyphenol mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
9.71
-
 4-hydroquinone mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
9.8
-
 2,6-dimethoxyphenol mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
10
-
 2,6-dimethoxyphenol mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
10.3
-
 4-hydroquinone wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
10.6
-
 2,6-dimethoxyphenol mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
10.9
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
11.4
-
 4-hydroquinone mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
11.6
-
 4-hydroquinone mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
12.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
13.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
13.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
13.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
14.6
-
 4-hydroquinone mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
15.6
-
 4-hydroquinone mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
16.1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
16.2
-
 4-hydroquinone mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
16.2
-
 guaiacol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
17.3
-
 2,6-dimethoxyphenol mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
17.5
-
 guaiacol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
19.2
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
19.2
-
 4-hydroquinone mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
20
-
 4-hydroquinone mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
20
-
 4-hydroquinone mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
20.4
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
20.4
-
 4-hydroquinone mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
22.7
-
 guaiacol wild-type, pH 3.5, 25°C Pleurotus eryngii
23.8
-
 guaiacol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
25.2
-
 4-hydroquinone mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
25.8
-
 4-hydroquinone mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
26.2
-
 guaiacol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
29.8
-
 guaiacol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
29.8
-
 2,6-dimethoxyphenol wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
29.9
-
 4-hydroquinone mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
30.6
-
 guaiacol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
33.3
-
 guaiacol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
34.8
-
 guaiacol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
35.1
-
 4-hydroquinone wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
35.2
-
 4-hydroquinone mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
47.6
-
 2,6-dimethoxyphenol mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
50.2
-
 2,6-dimethoxyphenol mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
56.6
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
61
-
 2,6-dimethoxyphenol mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
65.5
-
 4-hydroquinone mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
67
-
 2,6-dimethoxyphenol mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
70.6
-
 4-hydroquinone mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
72.9
-
 4-hydroquinone mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
74.5
-
 2,6-dimethoxyphenol mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
75
-
 catechol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
78
-
 catechol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
80
-
 catechol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
81
-
 catechol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
82.1
-
 4-hydroquinone mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
82.2
-
 4-hydroquinone mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
85
-
 catechol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
85.6
-
 4-hydroquinone mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
93
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
94
-
 catechol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
99
-
 catechol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
105.6
-
 catechol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
105.6
-
 guaiacol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
108
-
 4-hydroquinone mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
135.7
-
 catechol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
135.7
-
 guaiacol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
141
-
 2,6-dimethoxyphenol mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
146
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
154
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
162
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
164
-
 catechol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
164.8
-
 catechol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
164.8
-
 guaiacol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
165
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
185
-
 catechol wild-type, pH 3.5, 25°C Pleurotus eryngii
185.6
-
 catechol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
185.6
-
 guaiacol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
186
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
204
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
205
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
216
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
223
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
227
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
231.6
-
 2,6-dimethoxyphenol mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
235
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
259
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
293
-
 2,6-dimethoxyphenol mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
326
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.6
-
 guaiacol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
0.7
-
 guaiacol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1
-
 catechol mutant W164S, pH 3.5, 25°C Pleurotus eryngii
1.3
-
 guaiacol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
1.3
-
 2,6-dimethoxyphenol mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1.4
-
 guaiacol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
1.6
-
 guaiacol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
1.6
-
 guaiacol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
1.6
-
 guaiacol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
1.9
-
 2,6-dimethoxyphenol mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2
-
 guaiacol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
2
-
 guaiacol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
2
-
 guaiacol wild-type, pH 3.5, 25°C Pleurotus eryngii
2.45
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.53
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.6
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.8
-
 2,6-dimethoxyphenol wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
2.9
-
 2,6-dimethoxyphenol mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
3.48
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
3.8
-
 2,6-dimethoxyphenol mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
4.2
-
 guaiacol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
6.4
-
 2,6-dimethoxyphenol mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
7.8
-
 2,6-dimethoxyphenol mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
8.9
-
 4-hydroquinone mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
9.3
-
 guaiacol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
10.1
-
 catechol mutant E140G/P141G, pH 3.5, 25°C Pleurotus eryngii
14.5
-
 catechol mutant F142G, pH 3.5, 25°C Pleurotus eryngii
15
-
 catechol mutant P141G, pH 3.5, 25°C Pleurotus eryngii
15.1
-
 2,6-dimethoxyphenol mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
17.1
-
 guaiacol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
17.6
-
 guaiacol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
19.9
-
 catechol mutant K215G, pH 3.5, 25°C Pleurotus eryngii
20.1
-
 catechol mutant K215Q, pH 3.5, 25°C Pleurotus eryngii
20.7
-
 catechol mutant P76G, pH 3.5, 25°C Pleurotus eryngii
21.6
-
 catechol mutant K176G, pH 3.5, 25°C Pleurotus eryngii
22.6
-
 2,6-dimethoxyphenol mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
23.8
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
25.3
-
 4-hydroquinone mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
28.8
-
 catechol mutant E140G/P141G/K176G, pH 3.5, 25°C Pleurotus eryngii
29.2
-
 4-hydroquinone mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
31
-
 2,6-dimethoxyphenol mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
31.4
-
 4-hydroquinone mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
36.7
-
 catechol wild-type, pH 3.5, 25°C Pleurotus eryngii
38.4
-
 catechol mutant E140G, pH 3.5, 25°C Pleurotus eryngii
44.6
-
 2,6-dimethoxyphenol mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
48.4
-
 catechol mutant E140G/W164S/K176G, pH 3.5, 25°C Pleurotus eryngii
49.1
-
 4-hydroquinone wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
50.6
-
 4-hydroquinone mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
50.9
-
 catechol mutant K176D, pH 3.5, 25°C Pleurotus eryngii
51
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
55.6
-
 4-hydroquinone mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
59.9
-
 4-hydroquinone mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
67
-
 4-hydroquinone mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
70.7
-
 catechol mutant E140G/K176G, pH 3.5, 25°C Pleurotus eryngii
71
-
 2,6-dimethoxyphenol wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
87.2
-
 4-hydroquinone mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
91.3
-
 2,6-dimethoxyphenol mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
93
-
 2,6-dimethoxyphenol mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
96.8
-
 4-hydroquinone mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
97
-
 2,6-dimethoxyphenol mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
97
-
 4-hydroquinone mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
99
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215Q, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
100
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176D, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
132.8
-
 4-hydroquinone mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
152
-
 2,6-dimethoxyphenol mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
153
-
 2,6-dimethoxyphenol mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
165
-
 2,6-dimethoxyphenol mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
171
-
 2,6-dimethoxyphenol mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
171
-
 2,6-dimethoxyphenol mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
171
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
185
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
200
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant W164S, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
201
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
203.2
-
 4-hydroquinone mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
244
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K215G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
283
-
 2,6-dimethoxyphenol mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
368
-
 4-hydroquinone mutant E140G/P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
387
-
 4-hydroquinone mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
565
-
 4-hydroquinone mutant E140G/P141G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
600
-
 4-hydroquinone mutant K176D, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
600
-
 4-hydroquinone mutant K215G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
616
-
 4-hydroquinone mutant K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
656
-
 4-hydroquinone wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
659
-
 4-hydroquinone mutant K215Q, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
676
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
710
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
789
-
 4-hydroquinone mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
800
-
 4-hydroquinone mutant E140G/K176G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
800
-
 4-hydroquinone mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
882
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
1120
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
1600
-
 4-hydroquinone mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
2640
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant F142G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
2700
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) wild-type, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
2830
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P76G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
3010
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant P141G, high efficiency site, pH 3.5, 25°C Pleurotus eryngii
4090
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/W164S/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
5670
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/P141G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii
5680
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) mutant E140G/K176G, low efficiency site, pH 3.5, 25°C Pleurotus eryngii