Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pleurotus eryngii |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant M247F | Pleurotus eryngii |
Protein Variants | Comment | Organism |
---|---|---|
A260F | kinetics similar to wild-type | Pleurotus eryngii |
K264A | kinetics similar to wild-type | Pleurotus eryngii |
M247F | 92% decrease in efficiency for oxidizing Reactive Black 5 | Pleurotus eryngii |
M247L | kinetics similar to wild-type | Pleurotus eryngii |
N256D/R257D/A260F | unstable, complete loss of activity | Pleurotus eryngii |
R257A/A260F | 43% decrease in efficiency for oxidizing veratryl alcohol | Pleurotus eryngii |
R257D | 83% decrease in efficiency for oxidizing veratryl alcohol | Pleurotus eryngii |
R257K | 65% decrease in efficiency for oxidizing veratryl alcohol | Pleurotus eryngii |
R257L | 3-fold increase in Km value for veratryl alcohol | Pleurotus eryngii |
S158D | kinetics similar to wild-type | Pleurotus eryngii |
S158E | kinetics similar to wild-type | Pleurotus eryngii |
S158E/R257D | unstable, complete loss of activity | Pleurotus eryngii |
W164S | complete loss of activity | Pleurotus eryngii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant M247L | Pleurotus eryngii | |
0.0014 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant S158D | Pleurotus eryngii | |
0.0017 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant R257L | Pleurotus eryngii | |
0.0019 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant A260F | Pleurotus eryngii | |
0.0022 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant S158E | Pleurotus eryngii | |
0.0025 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant K264A | Pleurotus eryngii | |
0.0026 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant M247L | Pleurotus eryngii | |
0.0026 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant R257K | Pleurotus eryngii | |
0.0027 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant R257L | Pleurotus eryngii | |
0.0028 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant S158E | Pleurotus eryngii | |
0.003 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, wild-type | Pleurotus eryngii | |
0.0031 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant M247F | Pleurotus eryngii | |
0.0032 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant R257A/A260F | Pleurotus eryngii | |
0.0034 | - |
Reactive Black 5 | pH 3.5, 25°C, wild-type | Pleurotus eryngii | |
0.0035 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant M247F | Pleurotus eryngii | |
0.0036 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant R257K | Pleurotus eryngii | |
0.004 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant S158D | Pleurotus eryngii | |
0.0049 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant R257A/A260F | Pleurotus eryngii | |
0.0065 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant K264A | Pleurotus eryngii | |
0.009 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant A260F | Pleurotus eryngii | |
1.65 | - |
veratryl alcohol | pH 3.0, 25°C, mutant S158E | Pleurotus eryngii | |
4.11 | - |
veratryl alcohol | pH 3.0, 25°C, mutant M247F | Pleurotus eryngii | |
4.13 | - |
veratryl alcohol | pH 3.0, 25°C, wild-type | Pleurotus eryngii | |
5.11 | - |
veratryl alcohol | pH 3.0, 25°C, mutant A260F | Pleurotus eryngii | |
5.68 | - |
veratryl alcohol | pH 3.0, 25°C, mutant K264A | Pleurotus eryngii | |
5.96 | - |
veratryl alcohol | pH 3.0, 25°C, mutant S158D | Pleurotus eryngii | |
6.99 | - |
veratryl alcohol | pH 3.0, 25°C, mutant M247L | Pleurotus eryngii | |
13.5 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257A/A260F | Pleurotus eryngii | |
13.5 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257L | Pleurotus eryngii | |
14.2 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257K | Pleurotus eryngii | |
54.7 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257D | Pleurotus eryngii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Manganese | Km value Mn2+ is 0.181 mM for wild-type | Pleurotus eryngii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus eryngii | O94753 | - |
- |
Storage Stability | Organism |
---|---|
4°C, pH 4-7, wild-type is stable for 96 h | Pleurotus eryngii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Pleurotus eryngii | ? + H2O | - |
? | |
Reactive Black 5 + H2O2 | - |
Pleurotus eryngii | oxidized Reactive Black 5 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Pleurotus eryngii | veratraldehyde + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant M247F | Pleurotus eryngii | |
0.7 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant M247F | Pleurotus eryngii | |
2.3 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant R257K | Pleurotus eryngii | |
3.1 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant A260F | Pleurotus eryngii | |
3.3 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant M247L | Pleurotus eryngii | |
4.2 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant K264A | Pleurotus eryngii | |
4.3 | - |
veratryl alcohol | pH 3.0, 25°C, mutant M247F | Pleurotus eryngii | |
4.6 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant S158E | Pleurotus eryngii | |
4.9 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant M247L | Pleurotus eryngii | |
4.9 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant S158D | Pleurotus eryngii | |
5 | - |
veratryl alcohol | pH 3.0, 25°C, mutant M247L | Pleurotus eryngii | |
5.5 | - |
Reactive Black 5 | pH 3.5, 25°C, wild-type | Pleurotus eryngii | |
5.9 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant A260F | Pleurotus eryngii | |
7.5 | - |
veratryl alcohol | pH 3.0, 25°C, mutant A260F | Pleurotus eryngii | |
7.7 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant S158D | Pleurotus eryngii | |
8.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, wild-type | Pleurotus eryngii | |
8.2 | - |
veratryl alcohol | pH 3.0, 25°C, mutant K264A | Pleurotus eryngii | |
8.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant K264A | Pleurotus eryngii | |
9.1 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant R257A/A260F | Pleurotus eryngii | |
9.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant S158E | Pleurotus eryngii | |
9.5 | - |
veratryl alcohol | pH 3.0, 25°C, wild-type | Pleurotus eryngii | |
9.6 | - |
veratryl alcohol | pH 3.0, 25°C, mutant S158D | Pleurotus eryngii | |
9.6 | - |
veratryl alcohol | pH 3.0, 25°C, mutant S158E | Pleurotus eryngii | |
10.9 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257K | Pleurotus eryngii | |
11.6 | - |
Reactive Black 5 | pH 3.5, 25°C, mutant R257L | Pleurotus eryngii | |
11.8 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant R257K | Pleurotus eryngii | |
17.9 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257A/A260F | Pleurotus eryngii | |
18.6 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant R257A/A260F | Pleurotus eryngii | |
19.6 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257D | Pleurotus eryngii | |
22.2 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 3.5, 25°C, mutant R257L | Pleurotus eryngii | |
27.3 | - |
veratryl alcohol | pH 3.0, 25°C, mutant R257L | Pleurotus eryngii |