EC Number   |
Protein Variants |
Reference |
|---|
   3.4.17.23 | H505A |
1.5% of wild-type activity with (7-methoxycoumarin-4-yl)acetyl-APK-2,4-dinitrophenyl as substrate |
668550 |
| 3.4.17.23 | R514Q |
about 10% of wild-type activity with (7-methoxycoumarin-4-yl)acetyl-APK-2,4-dinitrophenyl as substrate |
668550 |
| 3.4.17.23 | more |
ACE2 overexpression leads to markedly increased myocyte volume, assessed in primary rabbit myocytes |
708684 |
| 3.4.17.23 | K481Q |
angiotensin I cleavage activity is 21% of wild-type activity, angiotensin II cleavage activity is 71.8% of wild-type activity |
692319 |
| 3.4.17.23 | R169Q |
angiotensin I cleavage activity is 5.2% of wild-type activity, angiotensin II cleavage activity is 1.1% of wild-type activity. The mutant enzyme does not show any activity with angiotensin I in the absence of chloride ions |
692319 |
| 3.4.17.23 | W271A |
angiotensin I cleavage activity is 5.3% of wild-type activity, angiotensin II cleavage activity is 0.9% of wild-type activity. Lacks any significant chloride sensitivity with the substrate angiotensin I |
692319 |
| 3.4.17.23 | R514Q |
angiotensin I cleavage activity is 52% of wild-type activity, angiotensin II cleavage activity is 179.3% of wild-type activity, enhancement of angiotensin II cleavage is a result of a 2.5-fold increase in Vmax compared with the wild-type |
692319 |
| 3.4.17.23 | R169QK481QR514Q |
angiotensin I cleavage activity is 53.2% of wild-type activity, angiotensin II cleavage activity is 203.4% of wild-type activity |
692319 |
| 3.4.17.23 | R169Q |
as active as wild-type enzyme with (7-methoxycoumarin-4-yl)acetyl-APK-2,4-dinitrophenyl as substrate |
668550 |
| 3.4.17.23 | more |
construction of a soluble truncated mutant enzyme lacking the transmembrane and cytosolic domains |
657967, 659147 |
