EC Number |
Protein Variants |
Reference |
---|
2.4.1.65 | D112E |
muation decreases activity of the enzyme and does not interfere with H-type 1/H-type 2 acceptors |
637646 |
2.4.1.65 | D125E |
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity |
693000 |
2.4.1.65 | D336A |
fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V |
637645 |
2.4.1.65 | E111D |
site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity |
693000 |
2.4.1.65 | FT3dc |
mutant where the cytoplasmic domain (Asp-2 to Trp-13) is deleted |
661236 |
2.4.1.65 | I126V |
site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity |
693000 |
2.4.1.65 | K73T |
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme |
693000 |
2.4.1.65 | K73T/E111D |
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme |
693000 |
2.4.1.65 | K73T/I75V/T87A |
insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme |
693000 |
2.4.1.65 | K73T/R110W |
site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme |
693000 |