EC Number |
Protein Variants |
Reference |
---|
2.4.1.65 | W111A |
alpha-1,4-activity is decreased to 10-20% of the wild type activity |
661839 |
2.4.1.65 | W124F |
alpha-1,4-activity is decreased to 17% of the wild type activity |
661839 |
2.4.1.65 | W124A |
alpha-1,4-activity is decreased to 20% of the wild type activity |
661839 |
2.4.1.65 | W111F |
alpha-1,4-activity is decreased to 42% of the wild type activity |
661839 |
2.4.1.65 | W124Y |
alpha-1,4-activity is decreased to 52% of the wild type activity |
661839 |
2.4.1.65 | W111Y |
alpha-1,4-activity is decreased to 58% of the wild type activity |
661839 |
2.4.1.65 | more |
construction of several chimeric mutants by domain swappig, overview |
693000 |
2.4.1.65 | D336A |
fucosyltransferase III mutant enzyme shows reduced activity with a variety of acceptors, 40fold reduction in activity for Fucalpha(1,2)Galbeta(1,3)GlcNAc. 4fold reduction affinity for GDP-fucose. The single amino acid site Asp336 of FucT III and Ala349 of FucT V constitutes the only difference in the sequence of FucT III and V over the final 210 COOH-terminal amino acid residues, impacts the acceptor substrate profiles of FucT III and FuvT V |
637645 |
2.4.1.65 | T87K/V89I/A101T |
insertion mutagenesis of FucT-V, the mutant shows altered substrate specificity compared to the wild-type enzyme |
693000 |
2.4.1.65 | K73T/I75V/T87A |
insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme |
693000 |