EC Number   |
Protein Variants |
Reference |
|---|
   6.5.1.8 | E371Q |
the mutant shows reduced activity compared to the wild type enzyme |
748760 |
| 6.5.1.8 | H168A |
almost complete loss of activity |
740694 |
| 6.5.1.8 | H168A |
the mutant shows about 48% of 5'-OH-RNAp ligation activity compared to the wild type enzyme |
748103 |
| 6.5.1.8 | H168N |
complete loss of activity |
740694 |
| 6.5.1.8 | H168Q |
complete loss of activity |
740694 |
| 6.5.1.8 | H185A |
activity is severely impaired |
740694 |
| 6.5.1.8 | H185A |
the mutant shows 5'-OH-RNAp ligation activity similar to the wild type enzyme |
748103 |
| 6.5.1.8 | H205A |
inactive for ligation reaction |
-, 741323 |
| 6.5.1.8 | H236A |
inactive for ligation reaction |
-, 741323 |
| 6.5.1.8 | H280A |
active, protein is able to complement a Saccharomyces cerevisiae Trl1 mutant |
740694 |
