EC Number   |
Protein Variants |
Reference |
|---|
   6.5.1.3 | S170A |
about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 8% of the adenylyltransferase activity observed with wild-type enzyme |
662241 |
| 6.5.1.3 | S185N |
mutation reduces nick sealing activity to less than 1% of the wild-type level |
676230 |
| 6.5.1.3 | S185T |
mutation reduces nick sealing activity to 12% of the wild-type level |
676230 |
| 6.5.1.3 | S272A |
about 50% of the ligation activity of wild-type enzyme |
662241 |
| 6.5.1.3 | S292N |
about 25% of the ligation activity of wild-type enzyme |
662241 |
| 6.5.1.3 | S701A |
lethal mutation |
676215 |
| 6.5.1.3 | T1001A |
lethal mutation |
676215 |
| 6.5.1.3 | T117A |
the mutant has ATP affinity comparable to the wild type enzyme. The mutation favors the reverse RNA adenylylation reaction to deadenylate the 5'-AMP from the RNA-adenylate, thereby inhibiting phosphodiester bond synthesis |
748784 |
| 6.5.1.3 | T163A |
mutant is dysfunctional in ligase adenylylation |
676230 |
| 6.5.1.3 | T163S |
mutation reduces nick sealing activity to 2% of the wild-type level |
676230 |
