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(deoxyribonucleotide)n
circular (deoxyribonucleotide)n
-
Substrates: -
Products: -
?
(ribonucleotide)n
circular (ribonucleotide)n
-
Substrates: -
Products: -
?
5'-adenylated cytidine 5',3'-bisphosphate + diguanosine tetraphosphate
Gp4GpCp + pCpGp4GpCp
Tequatrovirus T4
-
Substrates: -
Products: -
?
8-Bromoadenosine 5'-triphosphate + (ribonucleotide)n + (ribonucleotide)m
8-Bromoadenosine monophosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: -
Products: -
?
8-Sulfoadenosine 5'-triphosphate + (ribonucleotide)n + (ribonucleotide)m
8-Sulfoadenosinemonophosphate + phosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: -
Products: -
?
ATP + (2E,6E)-farnesyl diphosphate
(2E,6E)-farnesyl-triphospho(5')adenosine + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + (2E,6E)-farnesyl triphosphate
(2E,6E)-farnesyl-tetraphospho(5')adenosine + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + (deoxyribonucleotide)n
adenylyl-(deoxyribonucleotide)n + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n
adenylyl-(ribonucleotide)n + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
ATP + (RNA)n
?
-
Substrates: -
Products: -
?
ATP + 5'-aaaCCAGUC
AMP + diphosphate + ?
-
Substrates: RNA-DNA hybrid as substrate, chemical step is represented as irreversible, because reverse reaction, pyrophosphorolysis with formation of a triphosphate is 10000000-times slower than the forward reaction
Products: -
ir
ATP + 5'-phosphate termini of RNA
?
-
Substrates: -
Products: -
?
ATP + ATP
P1,P3-bis(5'-adenosyl) triphosphate + ?
Tequatrovirus T4
-
Substrates: -
Products: -
?
ATP + ATP
P1,P4-bis(5'-adenosyl) tetraphosphate + diphosphate
Tequatrovirus T4
-
Substrates: -
Products: -
?
ATP + clodronate
adenosine 5'-(beta,gamma-dichloromethylenetriphosphate) + ?
Tequatrovirus T4
-
Substrates: -
Products: -
?
ATP + dimethylallyl diphosphate
dimethylallyl-triphospho(5')adenosine + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + etidronate
?
Tequatrovirus T4
-
Substrates: weak activity
Products: -
?
ATP + geranyl diphosphate
geranyl-triphospho(5')adenosine + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + geranyl triphosphate
geranyl-tetraphospho(5')adenosine + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + isopentenyl diphosphate
adenosine 5'-isopentenyl triphosphate + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + isopentenyl triphosphate
isopentenyl-tetraphospho(5')adenosine + diphosphate
Tequatrovirus T4
-
Substrates: assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP
Products: -
ir
ATP + methylenebisphosphonate
adenosine 5'-(beta,gamma-methylenetriphosphate) + ?
Tequatrovirus T4
-
Substrates: -
Products: -
?
ATP + RNA + 5'-aaCCAGUC
AMP + diphosphate + ?
-
Substrates: DNA-RNA hybrid
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
ATPgammaS + ATP
P1,P4-bis(5'-adenosyl) tetraphosphate + thiodiphosphate
Tequatrovirus T4
-
Substrates: -
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
GTP + ATP
P1-(5'-adenosyl),P4-(5'-guanosyl) tetraphosphate + diphosphate
Tequatrovirus T4
-
Substrates: -
Products: -
?
ITP + (ribonucleotide)n + (ribonucleotide)m
IMP + phosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: -
Products: -
?
additional information
?
-
ATP + (ribonucleotide)n + (ribonucleotide)m
?
-
Substrates: possibly involved in posttranscriptional RNA editing process of mitochondrial transcripts
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
?
-
Substrates: possibly involved in posttranscriptional RNA editing process of mitochondrial transcripts
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: DraRnl seals 3'-OH/5'-PO4 RNA nicks in either a duplex RNA or an RNA:DNA hybrid, but it cannot seal 3'-OH/5'-PO4 DNA nicks
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: the L1 ligase is regioselective for formation of the biologically relevant 5' to 3' phosphodiester bond rather than a 5' to 2' bond
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: T4 RNA ligase 1 can efficiently adenylate DNA oligos with 5' phosphate and 3' blocking group
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: the TS2126 RNA ligase catalyzes both inter- and intra-molecular single-stranded DNA ligation to more than 50% completion in not more than a few hours at an elevated temperature, although favoring intramolecular ligation on RNA and single-stranded DNA substrates
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: Trl1 executes the end-healing and end-sealing steps of tRNA splicing, requires a 2'-PO4 end for tRNA splicing in vivo
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: ligates two substrate RNAs, promoting the attack of the 3'-hydroxyl of one substrate upon the 5'-triphosphate of the other substrate with release of diphosphate, reverse of ligation has been detected, but very inefficient, not saturable by soluble amounts of diphosphate
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
1910, 1986, 1989, 1990, 1992, 1998, 2000, 2001, 650653, 651051, 651061, 652387, 652429, 653234, 653369, 653679, 654015 Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: -
Products: -
r
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: converts linear molecules of polyriboadenylate to circular form by the intramolecular covalent linkage of the 5'-phosphate end to the 3'-hydroxyl terminus
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: formation of a phosphodiester bond between a 5'-PO4-terminated oligonucleotide and a 3'-OH terminated oligonucleotide. Intramolecular formation of single-stranded circles with longer oligonucleotides of the type pAp(Ap)nAOH, where n is about 15 or greater. Intermolecular joining of pAp(Ap)3AOH, where the 5'-PO4-terminated oligonucleotide is short enough to prevent apposition of its 3' and 5' ends, to UpUpUOH
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: cylization of poly(A)
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: the best substrate is [5'-P]polyriboadenylate
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: only the di-magnesium-coordinated form Mg2ATP0 reacts with the enzyme, forming the covalent complex E-AMP, the reverse reaction, ATP synthesis, occurs between the mono-magnesium-coordinated diphosphate from MgP2O72- and the enzyme-MgAMP complex
Products: -
r
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: catalyzes the transfer of AMP from the E-AMP complex to diverse compounds, generating the product indicated between parentheses, ATP(Ap4A), NTP (Ap4N), P3 or P4 (p4A or p5A), ADP (Ap3A)
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: participates in repair, splicing and editing pathways, reseals broken RNAs or alters their primary structure, catalyzes intramolecular and intermolecular RNA strand joining through ligase-adenylate and RNA adenylate intermediates
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: RNA-editing
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: neither dATP, GTP, CTP, nor UTP can substitute for ATP. RNA ligation at a 3'-OH/5'-PO4 nick in a double-stranded RNA or an RNA-DNA hybrid
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: the kinetic mechanism of single-turnover nick sealing by T4 Rnl2-AMP is explored by using a rapid mix-quench method and the effects of 3'-OH mispairs and base damage lesions on the rates of nick 5'-adenylylation and phosphodiester synthesis is determined. With respect to the sealing of perfectly paired nicks the rates of step 2 catalysis are rapid (9.5-17.9/sec) and similar in magnitude to the step 3 rates (7.9-32/sec). Rnl2 is kinetically sensitive to all 3'-OH base mispairs
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: poly(A) containing 5'-P-2,3-cyclic P termini
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA substrates containing a 5'-phosphate terminus at one end and either a 2',3'-cyclic phosphate or a 2'-phosphate terminus at the other end. In the latter case the reaction is about 30% as active as with the cyclic derivative
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: participates in an in vitro splicing of yeast transfer RNA precursors containing intervening sequences. Covalently ligates tRNA5' and 3' half-molecules produced by a yeast endonuclease
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: conversion of linear polyribonucleotides into covalently closed circles, formation of a 2'-phosphomonoester,3',5'-phosphodiester linkage
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, essential for proliferation of Trypanosoma brucei
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, posttranscriptional insertion and deletion of uridylate residues in mitochondrial transcripts
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, posttranscriptional process by which uridine residues are added and deleted from mitochondrial mRNAs
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, posttranscriptional RNA processing in which uridylate residues are inserted into and deleted from pre-mRNAs to create start and stop codons, also acts to reseal mRNAs cleaved at incorrect sites
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, unique U insertion and U deletion process, involves cycles of pre-mRNA cleavage, terminal U addition or U removal, and religation, band IV is the RNA editing ligase needed to seal in U deletion
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: the enzyme is essential for survival of both insect and bloodstream forms of the parasite
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: catalyzes RNA end-joining
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Tequatrovirus T4
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
Tequatrovirus T4
Substrates: -
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: at 20% the activity relative to ATP
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: acts on single-stranded or double-stranded DNA molecules. Acts on very small pieces of ribonucleic acid, with 40mers being the probable upper size limit. The minimum size of the 5'-moiety is a ribonucleoside 3',5'-bisphosphate
Products: -
?
dATP + (ribonucleotide)n + (ribonucleotide)m
dAMP + phosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
additional information
?
-
-
Substrates: the group I intron from cyanobacterium Anabaena sp. catalyzes phosphodiester bond formation using a triphosphate on the 5'-terminal nucleotide, much like protein polymerases and engineered ribozymes. In the process, this ribozyme forms a unique circular RNA that incorporates the exogenous guanosine cofactor added during self-splicing
Products: -
?
additional information
?
-
Substrates: the RNA ligase has a a strict requirement for RNA substrates with a 2'-phosphate terminus for the ligation of RNAs with 5'-phosphate and 3'-hydroxyl ends. RNA ligase forms a 2'-phosphomonoester-3',5'-phosphodiester junction
Products: -
?
additional information
?
-
-
Substrates: the RNA ligase has a a strict requirement for RNA substrates with a 2'-phosphate terminus for the ligation of RNAs with 5'-phosphate and 3'-hydroxyl ends. RNA ligase forms a 2'-phosphomonoester-3',5'-phosphodiester junction
Products: -
?
additional information
?
-
-
Substrates: RtcA is able of cyclizing a 2'-phosphate RNA end in high yield. The rate of 2'-phosphate cyclization by RtcA is five orders of magnitude slower than 3'-phosphate cyclization, notwithstanding that RtcA binds with similar affinity to RNA3'p and RNA2'p substrates
Products: -
?
additional information
?
-
-
Substrates: enzyme does not discriminate between RNA and DNA for phosphodiester bond formation
Products: -
?
additional information
?
-
-
Substrates: intramolecular ligation of 5'-PO4 single-strand RNA to form a covalently closed circular RNA molecule through ligase-adenylylate and RNA-adenylylate intermediates AppRNA. At the optimal temperature of 65°C, AppRNA is predominantly ligated to a circular product. At 35°C, phosphodiester bond formation is suppressed and the majority of the AppRNA is deadenylylated
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes an adenylation reaction that is specific for ATP and either ssDNA or RNA. It does not require synthesis of a template strand for adenylation. To achieve quantitative DNA adenylation, single turnover reaction condition with substrate to enzyme ratio 1:1 should be used
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl termini of single-stranded RNAs. It can also react with RNA with a 3'-phosphate end to generate a 2',3'-cyclic phosphate. The enzyme can additionally remove adenosine from the 3'-terminus of the RNA to produce 3'-deadenylated RNA
Products: -
?
additional information
?
-
-
Substrates: the enzyme is unreactive with a 5'-32P-labeled single-strand 18-mer pDNA substrate
Products: -
?
additional information
?
-
-
Substrates: the enzyme seals nicked 3'-OH/5'-PO4 duplexes in which the 3'-OH strand is RNA. The enzyme entails reaction with ATP to form a covalent enzyme-AMP intermediate, transfer of AMP to the nick 5'-PO4, and attack of the RNA 3'-OH on the adenylylated nick to form a 3'-5' phosphodiester
Products: -
?
additional information
?
-
Tequatrovirus T4
-
Substrates: joins single-stranded DNA molecules much less efficiently. Many of them approximate a well-defined consensus sequence
Products: -
?
additional information
?
-
Tequatrovirus T4
-
Substrates: efficient ligation of single-stranded DNAs and RNAs
Products: -
?
additional information
?
-
Tequatrovirus T4
-
Substrates: synthesizes circular hammerhead ribozymes from oligonucleotides, small RNA molecules resistant to circuarization by other means can be efficiently circularized on a preparative scale using a suitable DNA template
Products: -
?
additional information
?
-
Tequatrovirus T4
-
Substrates: no activity with alendronate nor pamidronate
Products: -
?
additional information
?
-
Tequatrovirus T4
-
Substrates: T4 RNA ligase possesses nick-joining activity in RNA/DNA hybrids against junctional matches/mismatches complementary to the RNA strand as a ligation template. When a junctional mismatch is present at the 30-terminal of RNA probe, the nick-joining activity of T4Rnl2 is inhibited
Products: -
?
additional information
?
-
-
Substrates: RNA-stimulated exchange reaction between ATP and diphosphate
Products: -
?
additional information
?
-
-
Substrates: wheat RNA ligase can be dissected into three isolated domain enzymes that are responsible for its core ligase, 5'-kinase, and 2',3'-cyclic phosphate 3'-phosphodiesterase activities, respectively. A side reaction on 5'-tri/diphosphate RNAs is dependent on ATP, a 2'-phosphate-3'-hydroxyl end, and the ligase domain. Two RNA molecules having 5'-hydroxyl and 2',3'-cyclic monophosphate groups are ligated almost stoichiometrically after separate conversion of respective terminal phosphate states into reactive ones
Products: -
?
additional information
?
-
-
Substrates: wheat RNA ligase contains 5'-hydroxyl kinase, 2',3'-cyclic phosphate 3'-phosphodiesterase, and 5'-phosphate 2'-phosphate-3'-hydroxyl RNA ligase activities
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + (ribonucleotide)n + (ribonucleotide)m
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
additional information
?
-
-
Substrates: the group I intron from cyanobacterium Anabaena sp. catalyzes phosphodiester bond formation using a triphosphate on the 5'-terminal nucleotide, much like protein polymerases and engineered ribozymes. In the process, this ribozyme forms a unique circular RNA that incorporates the exogenous guanosine cofactor added during self-splicing
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
?
-
Substrates: possibly involved in posttranscriptional RNA editing process of mitochondrial transcripts
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
?
-
Substrates: possibly involved in posttranscriptional RNA editing process of mitochondrial transcripts
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: the L1 ligase is regioselective for formation of the biologically relevant 5' to 3' phosphodiester bond rather than a 5' to 2' bond
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: Trl1 executes the end-healing and end-sealing steps of tRNA splicing, requires a 2'-PO4 end for tRNA splicing in vivo
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: -
Products: -
r
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
Substrates: -
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: catalyzes the transfer of AMP from the E-AMP complex to diverse compounds, generating the product indicated between parentheses, ATP(Ap4A), NTP (Ap4N), P3 or P4 (p4A or p5A), ADP (Ap3A)
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: participates in repair, splicing and editing pathways, reseals broken RNAs or alters their primary structure, catalyzes intramolecular and intermolecular RNA strand joining through ligase-adenylate and RNA adenylate intermediates
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
Tequatrovirus T4
-
Substrates: RNA-editing
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, essential for proliferation of Trypanosoma brucei
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, posttranscriptional insertion and deletion of uridylate residues in mitochondrial transcripts
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, posttranscriptional process by which uridine residues are added and deleted from mitochondrial mRNAs
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, posttranscriptional RNA processing in which uridylate residues are inserted into and deleted from pre-mRNAs to create start and stop codons, also acts to reseal mRNAs cleaved at incorrect sites
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: RNA editing, unique U insertion and U deletion process, involves cycles of pre-mRNA cleavage, terminal U addition or U removal, and religation, band IV is the RNA editing ligase needed to seal in U deletion
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: the enzyme is essential for survival of both insect and bloodstream forms of the parasite
Products: -
?
ATP + (ribonucleotide)n + (ribonucleotide)m
AMP + diphosphate + (ribonucleotide)n+m
-
Substrates: catalyzes RNA end-joining
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
-
Substrates: -
Products: -
?
ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-(ribonucleotide)m
(ribonucleotide)n+m + AMP + diphosphate
Tequatrovirus T4
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
Substrates: -
Products: -
?
ATP + [RNA ligase]-L-lysine
[RNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
Tequatrovirus T4
Substrates: -
Products: -
?
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DELTA1-105
-
reacts with ATP to form covalent protein-adenylate adducts,mutant protein retains RNA sealing activity
DELTA1-125
-
reacts with ATP to form covalent protein-adenylate adducts, mutant protein retains RNA sealing activity
DELTA1-135
-
does not reacts with ATP to form covalent protein-adenylate adducts, mutant enzyme is unable to seal RNA strands
E230D
-
mutation reduces nick sealing activity to 1% of the wild-type level
E230Q
-
mutation reduces nick sealing activity to less than 1% of the wild-type level
E278A
-
mutant enzyme retains adenylyltransferase and RNA ligase activities
E305D
-
mutation reduces nick sealing activity to 1% of the wild-type level
E305Q
-
mutation reduces nick sealing activity to 7% of the wild-type level
F281L
-
mutation reduces nick sealing activity to 5% of the wild-type level
H167A
-
mutant is dysfunctional in ligase adenylylation
H167N
-
mutation reduces nick sealing activity to 7% of the wild-type level
H167Q
-
mutation reduces nick sealing activity to less than 1% of the wild-type level
K165A
-
mutant is inert in enzyme-adenylate formation and nick-joining
K186A
-
mutant is dysfunctional in ligase adenylylation
K186Q
-
mutation reduces nick sealing activity to 1% of the wild-type level
K186R
-
mutation reduces nick sealing activity to 3% of the wild-type level
K326Q
-
mutation reduces nick sealing activity to less than 1% of the wild-type level
K326R
-
mutation reduces nick sealing activity to 3% of the wild-type level
S185N
-
mutation reduces nick sealing activity to less than 1% of the wild-type level
S185T
-
mutation reduces nick sealing activity to 12% of the wild-type level
T163A
-
mutant is dysfunctional in ligase adenylylation
T163S
-
mutation reduces nick sealing activity to 2% of the wild-type level
T163V
-
mutation reduces nick sealing activity to 30% of the wild-type level
E256A
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
E95A
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
E96A
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
F175A
the mutant forms traced amounts of ligase-AMP intermediates, with more than 40fold decrease in affinity for ATP
K73A
the mutant has ATP affinity comparable to the wild type enzyme
N99A
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
R104A
the mutant forms traced amounts of ligase-AMP intermediates, with more than 40fold decrease in affinity for ATP
R118A
the mutant forms traced amounts of ligase-AMP intermediates, with more than 40fold decrease in affinity for ATP
R275A
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
R278A
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
R76A
the mutation does not have a major effect on all three steps in the ligation reaction
T117A
the mutant has ATP affinity comparable to the wild type enzyme. The mutation favors the reverse RNA adenylylation reaction to deadenylate the 5'-AMP from the RNA-adenylate, thereby inhibiting phosphodiester bond synthesis
E256A
-
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
-
E96A
-
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
-
R278A
-
the mutation shows a moderate effects on step 1 adenylylation (5-20fold decrease in affinity for ATP) as compared to the wild type enzyme
-
D95A
-
the mutant shows RNA 3'-P guanylylation activity
E206A
-
the mutant shows strongly reduced activity compared to the wild type enzyme
K480A
-
the mutant shows 2fold increased activity compared to the wild type enzyme
D120A
Tequatrovirus T4
-
alanine-scanning mutagenesis
D135A
Tequatrovirus T4
-
about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 4% of the adenylyltransferase activity observed with wild-type enzyme
D244A
Tequatrovirus T4
-
site-directed mutagenesis
D292A
Tequatrovirus T4
-
no ligation activity
D292E
Tequatrovirus T4
-
about 10% of the ligation activity of wild-type enzyme
D308A
Tequatrovirus T4
-
about 70% of the ligation activity of wild-type enzyme
E139A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity
E185A
Tequatrovirus T4
-
site-directed mutagenesis
E204A
Tequatrovirus T4
-
substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method
E227A
Tequatrovirus T4
-
site-directed mutagenesis
E227D
Tequatrovirus T4
-
site-directed mutagenesis
E227Q
Tequatrovirus T4
-
site-directed mutagenesis
E295A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation
E296A
Tequatrovirus T4
-
about 10% of the ligation activity of wild-type enzyme
E299A
Tequatrovirus T4
-
about 80% of the ligation activity of wild-type enzyme
E29A
Tequatrovirus T4
-
about 90% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity
E300A
Tequatrovirus T4
-
about 50% of the ligation activity of wild-type enzyme
E34A
Tequatrovirus T4
-
alanine-scanning mutagenesis
E34D
Tequatrovirus T4
-
substitution mutant, introduced into the rnl2 gene by PCR
E34Q
Tequatrovirus T4
-
substitution mutant, introduced into the rnl2 gene by PCR
E63A
Tequatrovirus T4
-
about 80% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity
E81A
Tequatrovirus T4
-
site-directed mutagenesis
E99A
Tequatrovirus T4
-
alanine-scanning mutagenesis
F119A
Tequatrovirus T4
-
alanine-scanning mutagenesis
F76A
Tequatrovirus T4
-
site-directed mutagenesis
F77A
Tequatrovirus T4
-
site-directed mutagenesis
F77L
Tequatrovirus T4
-
site-directed mutagenesis
G102A
Tequatrovirus T4
-
site-directed mutagenesis
G228A
Tequatrovirus T4
-
site-directed mutagenesis
G55A
Tequatrovirus T4
-
site-directed mutagenesis
H250A
Tequatrovirus T4
-
pRNA circularization by the mutant enzyme is 6% of the wild-type rate
H37A
Tequatrovirus T4
-
substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method
H37D
Tequatrovirus T4
-
substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method
K107A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity
K117A
Tequatrovirus T4
-
mutation is lethal in vivo
K119A
Tequatrovirus T4
-
site-directed mutagenesis
K119Q
Tequatrovirus T4
-
site-directed mutagenesis
K119R
Tequatrovirus T4
-
site-directed mutagenesis
K189A
Tequatrovirus T4
-
alanine-scanning mutagenesis
K209A
Tequatrovirus T4
-
alanine-scanning mutagenesis
K225A
Tequatrovirus T4
-
substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method
K227A
Tequatrovirus T4
-
substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method
K240A
Tequatrovirus T4
-
site-directed mutagenesis
K240Q
Tequatrovirus T4
-
site-directed mutagenesis
K240R
Tequatrovirus T4
-
site-directed mutagenesis
K242A
Tequatrovirus T4
-
site-directed mutagenesis
K273A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation
K314A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation
K315A
Tequatrovirus T4
-
about 90% of the ligation activity of wild-type enzyme
K319A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation
K35A
Tequatrovirus T4
-
substitution mutation introduced into the ORFb y PCR with two-stage overlap extension method
K54A
Tequatrovirus T4
-
about 15% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity
K75A
Tequatrovirus T4
-
site-directed mutagenesis
K75Q
Tequatrovirus T4
-
site-directed mutagenesis
K75R
Tequatrovirus T4
-
site-directed mutagenesis
K99A
Tequatrovirus T4
-
site-directed mutagenesis
N184A
Tequatrovirus T4
-
site-directed mutagenesis
N309A
Tequatrovirus T4
-
about 70% of the ligation activity of wild-type enzyme
N40A
Tequatrovirus T4
-
alanine-scanning mutagenesis
N40D
Tequatrovirus T4
-
substitution mutant, introduced into the rnl2 gene by PCR
N40Q
Tequatrovirus T4
-
substitution mutant, introduced into the rnl2 gene by PCR
N40R
Tequatrovirus T4
-
substitution mutant, introduced into the rnl2 gene by PCR
N78A
Tequatrovirus T4
-
site-directed mutagenesis
Q106A
Tequatrovirus T4
-
about 20% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity
R155A
Tequatrovirus T4
-
about 35% of the ligation activity of wild-type enzyme, mutant enzyme displays 16% of the adenylyltransferase activity observed with wild-type enzyme
R166A
Tequatrovirus T4
-
site-directed mutagenesis
R182A
Tequatrovirus T4
-
site-directed mutagenesis
R221A
Tequatrovirus T4
-
about 60% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity
R266A
Tequatrovirus T4
-
no ligation activity
R266K
Tequatrovirus T4
-
about 50% of the ligation activity of wild-type enzyme
R266Q
Tequatrovirus T4
-
no ligation activity
R33A
Tequatrovirus T4
-
no ligation activity, mutant enzyme displays 3% of the adenylyltransferase activity observed with wild-type enzyme
R54A
Tequatrovirus T4
-
site-directed mutagenesis
R54K
Tequatrovirus T4
-
site-directed mutagenesis
R54Q
Tequatrovirus T4
-
site-directed mutagenesis
R55A
Tequatrovirus T4
-
alanine-scanning mutagenesis
R71A
Tequatrovirus T4
-
site-directed mutagenesis
S118A
Tequatrovirus T4
-
S118 strain is temperature sensitive, no growth at 37°C
S170A
Tequatrovirus T4
-
about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 8% of the adenylyltransferase activity observed with wild-type enzyme
S272A
Tequatrovirus T4
-
about 50% of the ligation activity of wild-type enzyme
S292N
Tequatrovirus T4
-
about 25% of the ligation activity of wild-type enzyme
W329A
Tequatrovirus T4
-
about as active as wild-type enzyme in RNA ligation
Y136A
Tequatrovirus T4
-
about 95% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity
Y5A
Tequatrovirus T4
-
about 10% of the RNA ligation activity of wild-type enzyme, mutant enzyme displays 39% of the adenylyltransferase activity observed with wild-type enzyme
K109R
-
site-directed mutagenesis
K86R
-
site-directed mutagenesis
E230A
-
mutant is defective in phosphodiester formation at a preadenylylated nick
E230A
-
mutant is dysfunctional in ligase adenylylation
E305A
-
mutant is defective in phosphodiester formation at a preadenylylated nick
E305A
-
mutant is dysfunctional in ligase adenylylation
F281A
-
mutant is defective in phosphodiester formation at a preadenylylated nick
F281A
-
mutant is dysfunctional in ligase adenylylation
G168A
-
mutant is defective in phosphodiester formation at a preadenylylated nick
G168A
-
mutant is dysfunctional in ligase adenylylation
L104A
Tequatrovirus T4
-
L104A strain is temperature sensitive, no growth at 37°C
L104A
Tequatrovirus T4
-
pRNA circularization by the mutant enzyme is 6% of the wild-type rate
S103A
Tequatrovirus T4
-
pRNA circularization by the mutant enzyme is 5% of the wild-type rate
S103A
Tequatrovirus T4
-
S103A strain is temperature sensitive, no growth at 37°C
additional information
-
expression of N-terminal amino acids 1-253 gives a protein defective in overall ligation but retaining the ability to form EpA intermediate, bind to pRNA and transfer AMP to pRNA, albeit less efficiently than wild-type ligase. Expression of amino acids 255-381 results in a protein that fails to form a detectable protein-RNA complex and does not support overall ligation or RNA circularization
additional information
Tequatrovirus T4
-
S121A, S124A, and H250 strains grow at all temperatures
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Maunders, M.J.
DNA and RNA ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3)
Methods Mol. Biol.
16
213-230
1993
Tequatrovirus T4
brenda
Pick, L.; Hurwitz, J.
Purification of wheat germ RNA ligase. I. Characterization of a ligase-associated 5'-hydroxyl polynucleotide kinase activity
J. Biol. Chem.
261
6684-6693
1986
Triticum aestivum
brenda
Pick, L.; Furneaux, H.; Hurwitz, J.
Purification of wheat germ RNA ligase. II. Mechanism of action of wheat germ RNA ligase
J. Biol. Chem.
261
6694-6704
1986
Triticum aestivum
brenda
Perkins, K.K.; Furneaux, H.; Hurwitz, J.
Isolation and characterization of an RNA ligase from HeLa cells
Proc. Natl. Acad. Sci. USA
82
684-688
1985
Homo sapiens
brenda
Rand, K.N.; Gait, M.J.
Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities
EMBO J.
3
397-402
1984
Tequatrovirus T4
brenda
Furneaux, H.; Pick, L.; Hurwitz, J.
Isolation and characterization of RNA ligase from wheat germ
Proc. Natl. Acad. Sci. USA
80
3933-3937
1983
Triticum aestivum
brenda
Gegenheimer, P.; Gabius, H.J.; Peebles, C.L.; Abelson, J.
An RNA ligase from wheat germ which participates in transfer RNA splicing in vitro
J. Biol. Chem.
258
8365-8373
1983
Triticum aestivum
brenda
Mei-Hao, H.; Wang, A.; Hui-Fen, H.
Purification of T4 RNA ligase by dextran blue-Sepharose 4B affinity chromatography
Anal. Biochem.
125
1-5
1982
Tequatrovirus T4
brenda
Dolganov, G.M.; Chestukhin, A.V.; Shemyakin, M.
A new procedure for the simultaneous large-scale purification of bacteriophage-T4-induced polynucleotide kinase, DNA ligase, RNA ligase and DNA polymerase
Eur. J. Biochem.
114
247-254
1981
Tequatrovirus T4
brenda
Konarska, M.; Filipowicz, W.; Domdey, H.; Gross, H.J.
Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ
Nature
293
112-116
1981
Triticum aestivum
brenda
Moseman McCoy, M.I.; Lubben, T.H.; Gumport, R.I.
The Purification of nuclease-free T4-RNA ligase
Biochim. Biophys. Acta
562
149-161
1979
Tequatrovirus T4
brenda
Sugiura, M.; Suzuki, M.; Ohtsuka, E.; Nishikawa, S.; Uemura, H.; Ikehara, M.
Purification of T4 RNA ligase by 2',5'-ADP Sepharose chromatography
FEBS Lett.
97
73-76
1979
Tequatrovirus T4
brenda
Last, J.A.; Anderson, W.F.
Purification and properties of bacteriophage T4-induced RNA ligase
Arch. Biochem. Biophys.
174
167-176
1976
Tequatrovirus T4
brenda
Silber, R.; Malathi, V.G.; Hurwitz, J.
Purification and properties of bacteriophage T4-induced RNA ligase
Proc. Natl. Acad. Sci. USA
69
3009-3013
1972
Tequatrovirus T4
brenda
Rusche, L.N.; Cruz-Reyes, J.; Piller, K.J.; Sollner-Webb, B.
Purification of a functional enzymatic editing complex from Trypanosoma brucei mitochondria
EMBO J.
16
4069-4081
1997
Trypanosoma brucei
brenda
Bakalara, N.; Simpson, A.M.; Simpson, L.
The Leishmania kinetoplast-mitochondrion contains terminal uridylyltransferase and RNA ligase activities
J. Biol. Chem.
264
18679-18686
1989
Leishmania tarentolae
brenda
Juodka, B.; Labeikyte, D.
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Branchiostoma floridae has separate healing and sealing enzymes for 5'-phosphate RNA ligation
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Das, U.; Shuman, S.
2-Phosphate cyclase activity of RtcA: a potential rationale for the operon organization of RtcA with an RNA repair ligase RtcB in Escherichia coli and other bacterial taxa
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Escherichia coli
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Tequatrovirus T4
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Pyrococcus horikoshii
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Tequatrovirus T4 (P32277)
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Unciuleac, M.C.; Goldgur, Y.; Shuman, S.
Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase
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Naegleria gruberi
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Unciuleac, M.C.; Shuman, S.
Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi
RNA
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Naegleria gruberi
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Methanothermobacter thermautotrophicus
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