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Protein Variants:
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EC Number
Protein Variants
Commentary
Reference
4.2.99.20
R124A
conserved among MenH proteins
702326
4.2.99.20
R168A
conserved among MenH proteins
702326
4.2.99.20
R90A
conserved among MenH proteins
702326
4.2.99.20
S86A
decreased activity
685225
4.2.99.20
S86A
kcat/Km is 1498fold lower than wild-type value
685225
4.2.99.20
S86A
part of the catalytically essential triad
702326
4.2.99.20
V152A
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
730042
4.2.99.20
V152G
residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity
730042
4.2.99.20
V152G/F153A
inactive
730042
4.2.99.20
W147A
conserved among MenH proteins
702326
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