4.2.99.20 D128A conserved among MenH proteins 702326 4.2.99.20 D210A decreased activity 685225 4.2.99.20 D210A kcat/Km is 9091fold lower than wild-type value 685225 4.2.99.20 D210A part of the catalytically essential triad 702326 4.2.99.20 F153A residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity 730042 4.2.99.20 H232A decreased activity 685225 4.2.99.20 H232A kcat/Km is 208333fold lower than wild-type value 685225 4.2.99.20 H232A part of the catalytically essential triad 702326 4.2.99.20 H232K part of the catalytically essential triad 702326 4.2.99.20 K212A conserved among MenH proteins 702326 4.2.99.20 R124A conserved among MenH proteins 702326 4.2.99.20 R168A conserved among MenH proteins 702326 4.2.99.20 R90A conserved among MenH proteins 702326 4.2.99.20 S86A decreased activity 685225 4.2.99.20 S86A kcat/Km is 1498fold lower than wild-type value 685225 4.2.99.20 S86A part of the catalytically essential triad 702326 4.2.99.20 V152A residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity 730042 4.2.99.20 V152G residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity 730042 4.2.99.20 V152G/F153A inactive 730042 4.2.99.20 W147A conserved among MenH proteins 702326 4.2.99.20 W147A/Y148A inactive 730042 4.2.99.20 W147F conserved among MenH proteins 702326 4.2.99.20 Y148A residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity 730042 4.2.99.20 Y148F residue involved in open-closed transition, mutation leads to large decrease in enzymatic activity 730042 4.2.99.20 Y85F conserved among MenH proteins 702326