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Literature summary for 4.2.99.20 extracted from
- Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli (2008), Biochemistry, 47, 3426-3434.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D210A | decreased activity | Escherichia coli |
| D210A | kcat/Km is 9091fold lower than wild-type value | Escherichia coli |
| H232A | decreased activity | Escherichia coli |
| H232A | kcat/Km is 208333fold lower than wild-type value | Escherichia coli |
| S86A | decreased activity | Escherichia coli |
| S86A | kcat/Km is 1498fold lower than wild-type value | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | pH 7.0, 37°C | Escherichia coli |  |
| 0.0083 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type at 37°C | Escherichia coli | |
| 0.0086 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, mutant enzyme H232A | Escherichia coli | |
| 0.0086 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | mutant H232A | Escherichia coli | |
| 0.0101 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type at 25°C | Escherichia coli | |
| 0.0191 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, wild-type enzyme | Escherichia coli | |
| 0.028 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, mutant enzyme S86A | Escherichia coli | |
| 0.028 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | mutant S86A at 25°C | Escherichia coli | |
| 0.118 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, mutant enzyme D210A | Escherichia coli | |
| 0.118 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | mutant D210A at 25°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | SHCHC synthase activity of MenH is not affected by the addition of a divalent ion (1 mM of Mg2+, Ba2+, Mn2+, Ca2+, Co2+, or Ni2+) | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25400 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | Escherichia coli | step in menaquinone biosynthesis | (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
| combination of Ni2+-affinity chromatography and size-exclusion chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | assay at 37°C, pH 7.0 | Escherichia coli | (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate + pyruvate | - |
? | |
| 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | - |
Escherichia coli | (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate | - |
? | |
| 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | step in menaquinone biosynthesis | Escherichia coli | (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
| monomer | 1 * 25400 | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase | - |
Escherichia coli |
| MenH | - |
Escherichia coli |
| SHCHC synthase | - |
Escherichia coli |
| YfbB | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, mutant enzyme D210A | Escherichia coli | |
| 0.29 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, mutant enzyme S86A | Escherichia coli | |
| 5.8 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, mutant enzyme H232A | Escherichia coli | |
| 147 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | 25°C, wild-type enzyme | Escherichia coli | |
| 167 | - |
5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate | pH 7.0, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | involved in menaquinone biosynthesis | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 67 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | mutant H232A | Escherichia coli | |
| 1500 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | mutant D210A at 25°C | Escherichia coli | |
| 10000 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | mutant S86A at 25°C | Escherichia coli | |
| 14000000 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type at 25°C | Escherichia coli | |
| 20000000 | - |
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate | wild-type at 37°C | Escherichia coli | |
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