EC Number   |
Protein Variants |
Reference |
|---|
   4.2.3.9 | E252Q |
mutant enzyme produces only (-)-germacrene A |
665301 |
| 4.2.3.9 | F112A |
expression of AS-F112A in Escherichia coli |
694515 |
| 4.2.3.9 | F112A |
residue 112 contributes to the stabilisation of the transition state following farnesyl cation |
682223 |
| 4.2.3.9 | F112A/F178A |
mutant is constructed to confirm the proposed roles of both F178 and F112 |
682223 |
| 4.2.3.9 | F178C |
mutant is constructed to distinguish between the importance of size and aromaticity residue 178 |
682223 |
| 4.2.3.9 | F178I |
mutant is constructed to distinguish between the importance of size and aromaticity residue 178 |
682223 |
| 4.2.3.9 | F178V |
mutation leads to the accumulation of the intermediate germacrene A, the production of selinenes and the linear alpha- and beta-farnesene |
682223 |
| 4.2.3.9 | F178V |
wild-type enzyme produces 92% aristolochene, 8% germacrene and a small amount of valencene. Mutant enzyme produces 10.8% aristolochene, 54.1% germacrene, 5.2% valencene, 5.7% alpha-selinene, 9.1% beta-selinine, 9.2% (E)-beta-farnesene and 2.7% (E,E)-alpha-farnesene. kcat is 1429fold lower than wild-type value, Km-value is 4.9fold higher than wild-type value |
664584 |
| 4.2.3.9 | F178W |
mutant is constructed to distinguish between the importance of size and aromaticity residue 178 |
682223 |
| 4.2.3.9 | F178Y |
mutant is constructed to distinguish between the importance of size and aromaticity residue 178 |
682223 |
