Literature summary for 4.2.3.9 extracted from

Forcat, S.; Allemann, R.K.
Dual role for phenylalanine 178 during catalysis by aristolochene synthase (2004), Chem. Commun. (Camb.), 2004, 2094-2095.

Search for more literature for 4.2.3.9

Protein Variants

Protein Variants	Comment	Organism
F178V	wild-type enzyme produces 92% aristolochene, 8% germacrene and a small amount of valencene. Mutant enzyme produces 10.8% aristolochene, 54.1% germacrene, 5.2% valencene, 5.7% alpha-selinene, 9.1% beta-selinine, 9.2% (E)-beta-farnesene and 2.7% (E,E)-alpha-farnesene. kcat is 1429fold lower than wild-type value, Km-value is 4.9fold higher than wild-type value	Penicillium roqueforti
F178Y	wild-type enzyme produces 92% aristolochene, 8% germacrene and a small amount of valencene. Mutant enzyme produces 86.4% aristolochene, 10.7% germacrene, and 2.7% valencene. kcat is 30fold lower than wild-type value, Km-value is 2.2fold higher than wild-type value	Penicillium roqueforti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0051	-	trans,trans-farnesyl diphosphate	wild-type enzyme, mutant enzyme F178Y	Penicillium roqueforti
0.0112	-	trans,trans-farnesyl diphosphate	wild-type enzyme, mutant enzyme F178V	Penicillium roqueforti

Organism

Organism	UniProt	Comment	Textmining
Penicillium roqueforti	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trans,trans-farnesyl diphosphate	-	Penicillium roqueforti	aristolochene + diphosphate + germacrene	wild-type enzyme produces 92% aristolochene, 8% germacrene and a small amount of valencene	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.000021	-	trans,trans-farnesyl diphosphate	wild-type enzyme, mutant enzyme F178V	Penicillium roqueforti
0.001	-	trans,trans-farnesyl diphosphate	wild-type enzyme, mutant enzyme F178Y	Penicillium roqueforti

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)