Cloned (Comment) | Organism |
---|---|
for expression in Escherichia coli BL21DE3 cells | Penicillium roqueforti |
Protein Variants | Comment | Organism |
---|---|---|
F112A | residue 112 contributes to the stabilisation of the transition state following farnesyl cation | Penicillium roqueforti |
F112A/F178A | mutant is constructed to confirm the proposed roles of both F178 and F112 | Penicillium roqueforti |
F178C | mutant is constructed to distinguish between the importance of size and aromaticity residue 178 | Penicillium roqueforti |
F178I | mutant is constructed to distinguish between the importance of size and aromaticity residue 178 | Penicillium roqueforti |
F178V | mutation leads to the accumulation of the intermediate germacrene A, the production of selinenes and the linear alpha- and beta-farnesene | Penicillium roqueforti |
F178W | mutant is constructed to distinguish between the importance of size and aromaticity residue 178 | Penicillium roqueforti |
F178Y | mutant is constructed to distinguish between the importance of size and aromaticity residue 178 | Penicillium roqueforti |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
farnesyl diphosphate | mutant F178I | Penicillium roqueforti | |
0.002 | - |
farnesyl diphosphate | mutant F178V | Penicillium roqueforti | |
0.0047 | - |
farnesyl diphosphate | mutant F178W | Penicillium roqueforti | |
0.0059 | - |
farnesyl diphosphate | mutant F178C | Penicillium roqueforti | |
0.0087 | - |
farnesyl diphosphate | wild-type | Penicillium roqueforti | |
0.0099 | - |
farnesyl diphosphate | mutant F178Y | Penicillium roqueforti | |
0.0197 | - |
farnesyl diphosphate | mutant F112A | Penicillium roqueforti | |
0.0463 | - |
farnesyl diphosphate | mutant F112A-178A | Penicillium roqueforti |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Penicillium roqueforti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | Penicillium roqueforti | - |
aristolochene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium roqueforti | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Penicillium roqueforti |
Renatured (Comment) | Organism |
---|---|
protein is extracted from inclusion bodies | Penicillium roqueforti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-trans,6-trans-farnesyl diphosphate | - |
Penicillium roqueforti | aristolochene + diphosphate | - |
? | |
farnesyl diphosphate | - |
Penicillium roqueforti | (+)-aristolochene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Penicillium roqueforti |
Synonyms | Comment | Organism |
---|---|---|
aristolochene synthase | - |
Penicillium roqueforti |
AS | - |
Penicillium roqueforti |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
activity assay | Penicillium roqueforti |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
farnesyl diphosphate | mutant F112A-178A | Penicillium roqueforti | |
0.0023 | - |
farnesyl diphosphate | mutant F112A | Penicillium roqueforti | |
0.0041 | - |
farnesyl diphosphate | mutant F178I | Penicillium roqueforti | |
0.0063 | - |
farnesyl diphosphate | mutant F178V | Penicillium roqueforti | |
0.0099 | - |
farnesyl diphosphate | mutant F178C | Penicillium roqueforti | |
0.052 | - |
farnesyl diphosphate | mutant F178Y | Penicillium roqueforti | |
0.079 | - |
farnesyl diphosphate | mutant F178W | Penicillium roqueforti | |
0.55 | - |
farnesyl diphosphate | wild-type | Penicillium roqueforti | |
1.4 | - |
farnesyl diphosphate | mutant F112A-178A | Penicillium roqueforti | |
2.3 | - |
farnesyl diphosphate | mutant F112A | Penicillium roqueforti | |
4.1 | - |
farnesyl diphosphate | mutant F178I | Penicillium roqueforti | |
6.3 | - |
farnesyl diphosphate | mutant F178V | Penicillium roqueforti | |
9.9 | - |
farnesyl diphosphate | mutant F178C | Penicillium roqueforti | |
52 | - |
farnesyl diphosphate | mutant F178Y | Penicillium roqueforti | |
79 | - |
farnesyl diphosphate | mutant F178W | Penicillium roqueforti | |
550 | - |
farnesyl diphosphate | wild-type | Penicillium roqueforti |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
activity assay | Penicillium roqueforti |