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Literature summary for 4.2.3.9 extracted from

  • Forcat, S.; Allemann, R.K.
    Stabilisation of transition states prior to and following eudesmane cation in aristolochene synthase (2006), Org. Biomol. Chem., 4, 2563-2567.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli BL21DE3 cells Penicillium roqueforti

Protein Variants

Protein Variants Comment Organism
F112A residue 112 contributes to the stabilisation of the transition state following farnesyl cation Penicillium roqueforti
F112A/F178A mutant is constructed to confirm the proposed roles of both F178 and F112 Penicillium roqueforti
F178C mutant is constructed to distinguish between the importance of size and aromaticity residue 178 Penicillium roqueforti
F178I mutant is constructed to distinguish between the importance of size and aromaticity residue 178 Penicillium roqueforti
F178V mutation leads to the accumulation of the intermediate germacrene A, the production of selinenes and the linear alpha- and beta-farnesene Penicillium roqueforti
F178W mutant is constructed to distinguish between the importance of size and aromaticity residue 178 Penicillium roqueforti
F178Y mutant is constructed to distinguish between the importance of size and aromaticity residue 178 Penicillium roqueforti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
farnesyl diphosphate mutant F178I Penicillium roqueforti
0.002
-
farnesyl diphosphate mutant F178V Penicillium roqueforti
0.0047
-
farnesyl diphosphate mutant F178W Penicillium roqueforti
0.0059
-
farnesyl diphosphate mutant F178C Penicillium roqueforti
0.0087
-
farnesyl diphosphate wild-type Penicillium roqueforti
0.0099
-
farnesyl diphosphate mutant F178Y Penicillium roqueforti
0.0197
-
farnesyl diphosphate mutant F112A Penicillium roqueforti
0.0463
-
farnesyl diphosphate mutant F112A-178A Penicillium roqueforti

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Penicillium roqueforti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate Penicillium roqueforti
-
aristolochene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Penicillium roqueforti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Penicillium roqueforti

Renatured (Commentary)

Renatured (Comment) Organism
protein is extracted from inclusion bodies Penicillium roqueforti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-trans,6-trans-farnesyl diphosphate
-
Penicillium roqueforti aristolochene + diphosphate
-
?
farnesyl diphosphate
-
Penicillium roqueforti (+)-aristolochene + diphosphate
-
?

Subunits

Subunits Comment Organism
monomer
-
Penicillium roqueforti

Synonyms

Synonyms Comment Organism
aristolochene synthase
-
Penicillium roqueforti
AS
-
Penicillium roqueforti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
activity assay Penicillium roqueforti

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
farnesyl diphosphate mutant F112A-178A Penicillium roqueforti
0.0023
-
farnesyl diphosphate mutant F112A Penicillium roqueforti
0.0041
-
farnesyl diphosphate mutant F178I Penicillium roqueforti
0.0063
-
farnesyl diphosphate mutant F178V Penicillium roqueforti
0.0099
-
farnesyl diphosphate mutant F178C Penicillium roqueforti
0.052
-
farnesyl diphosphate mutant F178Y Penicillium roqueforti
0.079
-
farnesyl diphosphate mutant F178W Penicillium roqueforti
0.55
-
farnesyl diphosphate wild-type Penicillium roqueforti
1.4
-
farnesyl diphosphate mutant F112A-178A Penicillium roqueforti
2.3
-
farnesyl diphosphate mutant F112A Penicillium roqueforti
4.1
-
farnesyl diphosphate mutant F178I Penicillium roqueforti
6.3
-
farnesyl diphosphate mutant F178V Penicillium roqueforti
9.9
-
farnesyl diphosphate mutant F178C Penicillium roqueforti
52
-
farnesyl diphosphate mutant F178Y Penicillium roqueforti
79
-
farnesyl diphosphate mutant F178W Penicillium roqueforti
550
-
farnesyl diphosphate wild-type Penicillium roqueforti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
activity assay Penicillium roqueforti