EC Number   |
Protein Variants |
Reference |
|---|
   3.4.21.46 | P43S |
can not induce cell survival |
698902 |
| 3.4.21.46 | R115C |
activity like wild type |
718398 |
| 3.4.21.46 | R157A |
catalytically inactive |
718398 |
| 3.4.21.46 | R202A |
putative interaction with Glu230 in factor B, cleavage of factor B reduced to 20%, activity against peptides threefold increased |
718398 |
| 3.4.21.46 | R202A |
site-directed mutagenesis, the mutation R202A removes the Arg202-Asp177 salt bridge, the mutant variant has enhanced activity towards artificial peptides compared to the wild-type enzyme and simultaneously displays active and inactive conformations of the active site. Ensemble refinement reveals pronounced disorder in the exosite loops for this enzyme variant, reminiscent of thrombin in the absence of the stabilizing Na+ ion, structure analysis |
731071 |
| 3.4.21.46 | R22I |
can not induce cell survival |
698902 |
| 3.4.21.46 | R22I/C25L |
has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells, has increased dimer to monomer ratio |
698902 |
| 3.4.21.46 | R22L |
can not induce cell survival |
698902 |
| 3.4.21.46 | R22L/C25L |
has biological activity comparable to that of the native protein in Ba/F3-VEGFR-2 cells, has increased dimer to monomer ratio |
698902 |
| 3.4.21.46 | S183A |
catalytically inactive |
718398 |
