BRENDA - Enzyme Database show
show all sequences of 3.4.21.46

Ensemble refinement shows conformational flexibility in crystal structures of human complement factor D

Forneris, F.; Burnley, B.T.; Gros, P.; Acta Crystallogr. Sect. D 70, 733-743 (2014)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
complement factor B
the enzyme factor D is activated by its substrate factor B through interactions outside the active site. The substrate-binding, or exosite, region displays a well defined and rigid conformation
Homo sapiens
Cloned(Commentary)
Commentary
Organism
transient expression of wild-type and mutant enzymes in HEK-293-E cells from pUPE.05.05 expression vectors, the recombinant proteins are secreted
Homo sapiens
Crystallization (Commentary)
Crystallization
Organism
wild-type enzyme, mutants R202A and S81Y/T198S/S199W, and enzyme mutant S183A in ternary complex with C3bB, hanging drop vapour diffusion technique, mixing of 10 mg/ml protein solution with reservoir solution consisting of 15% w/v PEG 6000, 50 mM MES-NaOH pH 6.0, 18°C, 6 days, X-ray diffraction structure determination and analysis at 1.8 A, 2.8 A, 2.0 A, and 3.5 A resolution, respectively, molecular replacement and ensemble refinement
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
R202A
site-directed mutagenesis, the mutation R202A removes the Arg202-Asp177 salt bridge, the mutant variant has enhanced activity towards artificial peptides compared to the wild-type enzyme and simultaneously displays active and inactive conformations of the active site. Ensemble refinement reveals pronounced disorder in the exosite loops for this enzyme variant, reminiscent of thrombin in the absence of the stabilizing Na+ ion, structure analysis
Homo sapiens
S183A
site-directed mutagenesis, structure analysis
Homo sapiens
S81Y/T198S/S199W
site-directed mutagenesis, the mutation causes steric hindrance of Trp199 resulting in pronounced rearrangement of the proteinase active-site region, structure analysis
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
human factor D is a self-inhibited thrombin-like serine proteinase. Ser199 of the self-inhibitory loop blocks the formation of the canonical active configurations
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
complement component C3bB + H2O
Homo sapiens
-
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
P00746
-
-
Purification (Commentary)
Commentary
Organism
recombinant soluble wild-type and mutant enzymes from HEK-293-E cells by cation exchange chromatography and gel filtration
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
complement component C3bB + H2O
-
731071
Homo sapiens
?
-
-
-
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
complement factor B
the enzyme factor D is activated by its substrate factor B through interactions outside the active site. The substrate-binding, or exosite, region displays a well defined and rigid conformation
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
transient expression of wild-type and mutant enzymes in HEK-293-E cells from pUPE.05.05 expression vectors, the recombinant proteins are secreted
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
wild-type enzyme, mutants R202A and S81Y/T198S/S199W, and enzyme mutant S183A in ternary complex with C3bB, hanging drop vapour diffusion technique, mixing of 10 mg/ml protein solution with reservoir solution consisting of 15% w/v PEG 6000, 50 mM MES-NaOH pH 6.0, 18°C, 6 days, X-ray diffraction structure determination and analysis at 1.8 A, 2.8 A, 2.0 A, and 3.5 A resolution, respectively, molecular replacement and ensemble refinement
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R202A
site-directed mutagenesis, the mutation R202A removes the Arg202-Asp177 salt bridge, the mutant variant has enhanced activity towards artificial peptides compared to the wild-type enzyme and simultaneously displays active and inactive conformations of the active site. Ensemble refinement reveals pronounced disorder in the exosite loops for this enzyme variant, reminiscent of thrombin in the absence of the stabilizing Na+ ion, structure analysis
Homo sapiens
S183A
site-directed mutagenesis, structure analysis
Homo sapiens
S81Y/T198S/S199W
site-directed mutagenesis, the mutation causes steric hindrance of Trp199 resulting in pronounced rearrangement of the proteinase active-site region, structure analysis
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
human factor D is a self-inhibited thrombin-like serine proteinase. Ser199 of the self-inhibitory loop blocks the formation of the canonical active configurations
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
complement component C3bB + H2O
Homo sapiens
-
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant soluble wild-type and mutant enzymes from HEK-293-E cells by cation exchange chromatography and gel filtration
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
complement component C3bB + H2O
-
731071
Homo sapiens
?
-
-
-
?
General Information
General Information
Commentary
Organism
additional information
the substrate-binding, or exosite, region displays a well defined and rigid conformation. A salt bridge between Arg202 on the self-inhibitory loop and Asp177 locks the S1 pocket and rigidifies the exosite. Asp-His-Ser catalytic site. The enzyme exhibits conformational dynamics like thrombin, but unlike in thrombin a mechanism has evolved in factor D that locks the unbound native state into an ordered inactive conformation via the self-inhibitory loop. The Asp-His-Ser catalytic site of the enzyme is in an enzymatically active conformation induced by substrate binding at the exosite
Homo sapiens
physiological function
human factor D is a self-inhibited thrombin-like serine proteinase that is critical for amplification of the complement immune response
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
additional information
the substrate-binding, or exosite, region displays a well defined and rigid conformation. A salt bridge between Arg202 on the self-inhibitory loop and Asp177 locks the S1 pocket and rigidifies the exosite. Asp-His-Ser catalytic site. The enzyme exhibits conformational dynamics like thrombin, but unlike in thrombin a mechanism has evolved in factor D that locks the unbound native state into an ordered inactive conformation via the self-inhibitory loop. The Asp-His-Ser catalytic site of the enzyme is in an enzymatically active conformation induced by substrate binding at the exosite
Homo sapiens
physiological function
human factor D is a self-inhibited thrombin-like serine proteinase that is critical for amplification of the complement immune response
Homo sapiens
Other publictions for EC 3.4.21.46
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732521
Cresci
Alternative complement pathway ...
Mus musculus, Mus musculus C57BL/6
Mol. Immunol.
64
9-17
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
141
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
731071
Forneris
Ensemble refinement shows conf ...
Homo sapiens
Acta Crystallogr. Sect. D
70
733-743
2014
1
-
1
1
3
-
1
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
3
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
732272
Degn
The pro-Factor D cleaving acti ...
Mus musculus
J. Immunol.
192
5448-5449
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
732044
Katschke
Inhibiting alternative pathway ...
Homo sapiens, Macaca fascicularis
J. Biol. Chem.
287
12886-12892
2012
-
2
-
2
-
-
2
-
-
-
-
2
-
3
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
707940
Ahmad
Platelet-derived growth factor ...
Homo sapiens
Breast Cancer Res. Treat.
126
15-25
2011
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
1
1
2
-
-
708769
Hu
Deguelin-an inhibitor to tumor ...
Homo sapiens
Int. J. Cancer
127
2455-2466
2010
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
709321
Takahashi
Essential role of mannose-bind ...
Mus musculus
J. Exp. Med.
207
29-37
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718398
Forneris
Structures of C3b in complex w ...
Homo sapiens
Science
330
1816-1820
2010
-
-
1
1
6
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695580
Li
Vascular endothelial growth fa ...
Homo sapiens
Anat. Rec. (Hoboken)
292
562-569
2009
-
1
-
-
-
-
-
-
2
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698902
Toivanen
Novel vascular endothelial gro ...
Homo sapiens
J. Biol. Chem.
284
16037-1648
2009
-
-
1
-
13
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
13
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699302
Kinnunen
Baculovirus is an efficient ve ...
Homo sapiens
J. Gene Med.
11
382-389
2009
1
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708131
Glasgow
Serum vascular endothelial gro ...
Homo sapiens
Chest
135
1293-1300
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
708370
Ming
Interleukin 7/interleukin 7 re ...
Homo sapiens
Eur. J. Cancer
45
866-873
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
708533
Kong
An immune responsive complemen ...
Paralichthys olivaceus
Fish Shellfish Immunol.
27
486-492
2009
-
-
-
-
-
-
-
-
-
-
1
-
-
8
-
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
710586
Deng
Vascular endothelial growth fa ...
Homo sapiens
Surgery
146
896-905
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
697096
Schaefer
Regulation of vascular endothe ...
Homo sapiens
Cancer Res.
68
457-466
2008
1
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698194
Liu
Identification of platelet-der ...
Homo sapiens
Hum. Pathol.
39
393-402
2008
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683539
Luginbuehl
Complement factor D, albumin, ...
Homo sapiens
Infect. Immun.
75
1771-1777
2007
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668074
Sprong
Deficient alternative compleme ...
Homo sapiens
Blood
107
4865-4870
2006
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683526
Lovoll
Maternal transfer of complemen ...
Oncorhynchus mykiss
Immunogenetics
58
168-179
2006
-
-
-
-
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
6
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
6
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669356
Harris
Molecular dissection of intera ...
Homo sapiens
J. Biol. Chem.
280
2569-2578
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668289
Oda
Analysis of protein absorbed b ...
Homo sapiens
Clin. Chim. Acta
342
155-160
2004
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
670101
Elliott
Effects of complement factor D ...
Mus musculus
Kidney Int.
65
129-138
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670498
Recinos
Liver gene expression associat ...
Mus musculus
Physiol. Genomics
19
131-142
2004
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651513
Kitano
Synthesis of factor D by norma ...
Homo sapiens
Int. Arch. Allergy Immunol.
122
299-302
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36585
Jing
Structural basis of profactor ...
Homo sapiens
EMBO J.
18
804-814
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36589
Jing
Structures of native and compl ...
Homo sapiens
J. Mol. Biol.
282
1061-1081
1998
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36588
Cole
Structure of diisopropyl fluor ...
Homo sapiens
Acta Crystallogr. Sect. D
53
143-150
1997
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36586
Balke
Inhibition of degranulation of ...
Homo sapiens
FEBS Lett.
371
300-302
1995
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36587
Kim
Crystal structure of a complem ...
Homo sapiens
J. Biol. Chem.
270
24399-24405
1995
-
-
-
1
3
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
36592
Volanakis
Purification and properties of ...
Homo sapiens
Methods Enzymol.
223
82-97
1993
-
-
1
1
-
-
-
-
-
-
1
-
-
2
-
1
1
-
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
1
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36590
Catana
Purification of human compleme ...
Homo sapiens
J. Immunol. Methods
138
265-271
1991
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
7
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
7
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36591
Miyata
Molecular and functional ident ...
Homo sapiens
Mol. Immunol.
27
637-644
1990
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36579
Harper
Reaction of serine proteases w ...
Homo sapiens
Biochemistry
24
1831-1841
1985
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36580
Davis III
-
Factor D of the alternative co ...
Homo sapiens
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
5
549-553
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36581
Reid
Preparation of human factor D ...
Homo sapiens
Methods Enzymol.
80
134-143
1981
-
-
-
-
-
1
1
-
-
-
-
1
-
2
-
-
1
-
-
-
1
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36582
Davies
Human factor D of the alternat ...
Homo sapiens
Biochemistry
18
5082-5087
1979
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36583
Lesavre
The alternative pathway C3/C5 ...
Homo sapiens
J. Immunol.
123
529-534
1979
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
36584
Volanakis
Human factor D of the alternat ...
Homo sapiens
J. Immunol.
119
337-342
1977
-
-
-
-
-
-
1
-
-
-
1
-
-
2
-
-
1
-
-
1
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
1
1
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-