EC Number |
Protein Variants |
Reference |
---|
3.1.1.45 | C151S |
site-directed mutagenesis, almost inactive mutant |
714312 |
3.1.1.45 | C60S |
no reduction in activity |
94355, 94364 |
3.1.1.45 | D107A |
mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity |
702072 |
3.1.1.45 | D107A/H111A |
mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity |
702072 |
3.1.1.45 | D171N |
no detectable activity |
94356 |
3.1.1.45 | D198N |
site-directed mutagenesis, almost inactive mutant |
714312 |
3.1.1.45 | E294A |
mutation of a residue in the putative metal-binding site of trans-DLH results in a drastic decrease in activity |
702072 |
3.1.1.45 | E36A |
no detectable activity |
94356 |
3.1.1.45 | E36A/C123S |
no activity activity towards alpha-naphthyl acetate |
-, 671042 |
3.1.1.45 | E36D |
104% activity towards alpha-naphthyl acetate compared with the wild type enzyme |
-, 671042 |