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Literature summary for 3.1.1.45 extracted from

  • Cheah, E.; Austin, C.; Ashley, G.W.; Ollis, D.
    Substrate-induced activation of dienelactone hydrolase: an enzyme with a naturally occurring Cys-His-Asp triad (1993), Protein Eng., 6, 575-583.
    View publication on PubMed
Search for more literature for 3.1.1.45

Crystallization (Commentary)

Crystallization (Comment) Organism
inhibitor, dienelactam, bound to DLH C123S Pseudomonas knackmussii

Protein Variants

Protein Variants Comment Organism
C123S maximal activity 20% that of the wild type protein Pseudomonas knackmussii
D171N no detectable activity Pseudomonas knackmussii
E36A no detectable activity Pseudomonas knackmussii

Inhibitors

Inhibitors Comment Organism Structure
dienelactam inhibitor binding studies Pseudomonas knackmussii
additional information no inactivation by iodoacetamide, iodoacetate, chloroacetamide Pseudomonas knackmussii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-carboxymethylenebut-2-en-4-olide + H2O Pseudomonas knackmussii the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas knackmussii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3-chlorobenzoate-grown cells Pseudomonas knackmussii
C123S DLH Pseudomonas knackmussii

Reaction

Reaction Comment Organism Reaction ID
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate mechanism Pseudomonas knackmussii
a
4-carboxymethylenebut-2-en-4-olide + H2O = 4-oxohex-2-enedioate the catalytic triad Cys123, His202 and Asp171 is a hybrid of the standard triads found in cysteine: Cys-His-Asn and serine: Ser-His-Asp proteases Pseudomonas knackmussii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxymethylenebut-2-en-4-olide + H2O the enzyme is one of the enzymes of the halocatechol branch of the beta-ketoadipate pathway, a complex set of catabolic reactions used by bacteria for utilization of aromatic compounds Pseudomonas knackmussii ?
-
 ?
cis-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?
trans-4-carboxymethylenebut-2-en-4-olide + H2O
-
 Pseudomonas knackmussii 4-oxohex-2-enedioate
-
 ?

Subunits

Subunits Comment Organism
monomer alpha,beta protein Pseudomonas knackmussii

Synonyms

Synonyms Comment Organism
DLH
-
 Pseudomonas knackmussii