EC Number   |
Protein Variants |
Reference |
|---|
   2.7.7.7 | D1124E |
mutant enzyme with reduced polymerization activity, polymerization activity is 43% compared to wild-type activity, |
722619 |
| 2.7.7.7 | D1124N |
mutant enzyme with reduced polymerization activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type |
722619 |
| 2.7.7.7 | D112A/E114A |
the mutant T4 DNA polymerases is defective in 3'-5' exonuclease activity and has a 1000fold increase in the development of spontaneous mutations compared to wild type polymerase |
703192 |
| 2.7.7.7 | D115A/E116A |
catalytically inactive |
705693 |
| 2.7.7.7 | D171A |
96% loss of DNA polymerase activity |
726027 |
| 2.7.7.7 | D189G |
impaired for extension step of TLS |
761958 |
| 2.7.7.7 | D190A |
retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity |
643652 |
| 2.7.7.7 | D198A/E200A |
site-directed mutagenesis in the exonuclease domain resulting in loss of exonuclease activity |
723108 |
| 2.7.7.7 | D215A |
3'-5'-exonuclease inactive mutant enzyme |
721612 |
| 2.7.7.7 | D219A |
the mutant T4 DNA polymerases is defective in 3'-5' exonuclease activity and has a 1000fold increase in the development of spontaneous mutations compared to wild type polymerase |
703192 |
