Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
D1122A | loss of polymerization activity | Pyrococcus horikoshii |
D1122E | mutant enzyme with reduced polymerization activity, polymerization activity is 15% compared to wild-type activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type | Pyrococcus horikoshii |
D1124A | loss of polymerization activity | Pyrococcus horikoshii |
D1124E | mutant enzyme with reduced polymerization activity, polymerization activity is 43% compared to wild-type activity, | Pyrococcus horikoshii |
D1124N | mutant enzyme with reduced polymerization activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type | Pyrococcus horikoshii |
G1123A | mutant enzyme has 13% of the activity of the wild type enzyme | Pyrococcus horikoshii |
G1123R | mutant enzyme has 0.8% of the activity of the wild type enzyme | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal magnesium concentration is 17.5 mM | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
2 * 90000 + 2 * 140000, SDS-PAGE | Pyrococcus horikoshii |
140000 | - |
2 * 90000 + 2 * 140000, SDS-PAGE | Pyrococcus horikoshii |
421000 | - |
gel filtration | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O57863 and O57861 | O57863: small subunit, O57861: large subunit | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Pyrococcus horikoshii | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 90000 + 2 * 140000, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
DP1Pho | small subunit | Pyrococcus horikoshii |
DP2Pho | large subunit | Pyrococcus horikoshii |
PH0121 | gene name, large subunit | Pyrococcus horikoshii |
PH0123 | gene name, small subunit | Pyrococcus horikoshii |
PolDPho | - |
Pyrococcus horikoshii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
60 min, 50% loss of activity | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Pyrococcus horikoshii |