Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Shen, Y.; Musti, K.; Hiramoto, M.; Kikuchi, H.; Kawarabayashi, Y.; Matsui, I.
    Invariant Asp-1122 and Asp-1124 are essential residues for polymerization catalysis of family D DNA polymerase from Pyrococcus horikoshii (2001), J. Biol. Chem., 276, 27376-27383.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
D1122A loss of polymerization activity Pyrococcus horikoshii
D1122E mutant enzyme with reduced polymerization activity, polymerization activity is 15% compared to wild-type activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type Pyrococcus horikoshii
D1124A loss of polymerization activity Pyrococcus horikoshii
D1124E mutant enzyme with reduced polymerization activity, polymerization activity is 43% compared to wild-type activity, Pyrococcus horikoshii
D1124N mutant enzyme with reduced polymerization activity, 3'-5' exonuclease activity remains, the mutant has lower Mg2+ affinity than does the wild-type Pyrococcus horikoshii
G1123A mutant enzyme has 13% of the activity of the wild type enzyme Pyrococcus horikoshii
G1123R mutant enzyme has 0.8% of the activity of the wild type enzyme Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal magnesium concentration is 17.5 mM Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
2 * 90000 + 2 * 140000, SDS-PAGE Pyrococcus horikoshii
140000
-
2 * 90000 + 2 * 140000, SDS-PAGE Pyrococcus horikoshii
421000
-
gel filtration Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O57863 and O57861 O57863: small subunit, O57861: large subunit
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Pyrococcus horikoshii diphosphate + DNAn+1
-
?

Subunits

Subunits Comment Organism
heterotetramer 2 * 90000 + 2 * 140000, SDS-PAGE Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
DP1Pho small subunit Pyrococcus horikoshii
DP2Pho large subunit Pyrococcus horikoshii
PH0121 gene name, large subunit Pyrococcus horikoshii
PH0123 gene name, small subunit Pyrococcus horikoshii
PolDPho
-
Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
60 min, 50% loss of activity Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Pyrococcus horikoshii