EC Number |
Protein Variants |
Reference |
---|
2.3.1.286 | I117F |
the mutant is nearly as active as the wild type protein (about 75% activity) |
751549 |
2.3.1.286 | I117H |
inactive |
751549 |
2.3.1.286 | I117L |
the mutant retains approximately 25% of the activity of the wild type protein |
751549 |
2.3.1.286 | I117V |
the mutant is nearly as active as the wild type protein (about 65% activity) |
751549 |
2.3.1.286 | I117W |
inactive |
751549 |
2.3.1.286 | I117Y |
inactive |
751549 |
2.3.1.286 | E78A |
site-directed mutagenesis, mutation of the conserved catalytic amino acid prevents PhnO-dependent acetylation |
757557 |
2.3.1.286 | F70A |
site-directed mutagenesis, mutation of the conserved catalytic amino acid reduces YiaC-dependent acetylation compared to wild-type. Overexpression of YiaC YF70A inhibits cell migration similarly to overexpression of wild-type YiaC |
757557 |
2.3.1.286 | more |
generation and analysis of the DELTAackA pta yfiQ mutant lacking the two known mechanisms of protein acetylation, but residual acetylation remains |
757557 |
2.3.1.286 | Y115A |
site-directed mutagenesis, mutation of the conserved catalytic amino acid prevents YiaC-dependent acetylation. Mutant YiaC Y115A is unable to inhibit cell migration in contrast to the wild-type enzyme |
757557 |